Spencer Daniel, Elliott Gregor, Morgan Hywel
Faculty of Physical Sciences and Engineering, and Institute for Life Sciences, University of Southampton, Southampton, Hampshire SO17 1BJ, UK.
Lab Chip. 2014 Aug 21;14(16):3064-73. doi: 10.1039/c4lc00224e.
We describe a sheath-less micro-cytometer that measures four different parameters, namely fluorescence, large angle side scatter and dual frequency electrical impedance (electrical volume and opacity). The cytometer was benchmarked using both size and fluorescent bead standards and demonstrates excellent size accuracy (CVs ≤ 2.1%), sensitivity and dynamic range (3.5 orders of magnitude) at sample flow rates of 80 μL per minute. The cytometer was evaluated by analysing human blood, and a four part differential leukocyte assay for accurate CD4+ T-cell enumeration was demonstrated. The integration of impedance, fluorescence and side scatter into a single miniature cytometer platform provides the core information content of a classical cytometer in a highly compact, simple, portable and low cost format.
我们描述了一种无鞘微细胞仪，它可以测量四个不同参数，即荧光、大角度侧向散射和双频电阻抗（电体积和不透明度）。该细胞仪使用尺寸和荧光珠标准进行了基准测试，在每分钟80微升的样品流速下，显示出出色的尺寸准确性（变异系数≤2.1%）、灵敏度和动态范围（3.5个数量级）。通过分析人体血液对该细胞仪进行了评估，并展示了用于准确计数CD4+T细胞的四分类白细胞分析。将阻抗、荧光和侧向散射集成到单个微型细胞仪平台中，以高度紧凑、简单、便携和低成本的形式提供了经典细胞仪的核心信息内容。
