Department of Biomedical Engineering, Chang Gung University, Taoyuan 33302, Taiwan.
Department of Radiation Oncology, Linkou Chang Gung Memorial Hospital, Taoyuan 33305, Taiwan.
Biosensors (Basel). 2023 Jan 3;13(1):83. doi: 10.3390/bios13010083.
Electrical impedance biosensors are powerful and continuously being developed for various biological sensing applications. In this line, the sensitivity of impedance biosensors embedded with microfluidic technologies, such as sheath flow focusing, dielectrophoretic focusing, and interdigitated electrode arrays, can still be greatly improved. In particular, reagent consumption reduction and analysis time-shortening features can highly increase the analytical capabilities of such biosensors. Moreover, the reliability and efficiency of analyses are benefited by microfluidics-enabled automation. Through the use of mature microfluidic technology, complicated biological processes can be shrunk and integrated into a single microfluidic system (e.g., lab-on-a-chip or micro-total analysis systems). By incorporating electrical impedance biosensors, hand-held and bench-top microfluidic systems can be easily developed and operated by personnel without professional training. Furthermore, the impedance spectrum provides broad information regarding cell size, membrane capacitance, cytoplasmic conductivity, and cytoplasmic permittivity without the need for fluorescent labeling, magnetic modifications, or other cellular treatments. In this review article, a comprehensive summary of microfluidics-based impedance biosensors is presented. The structure of this article is based on the different substrate material categorizations. Moreover, the development trend of microfluidics-based impedance biosensors is discussed, along with difficulties and challenges that may be encountered in the future.
基于阻抗的生物传感器是一种强大的工具，正在被不断开发，以应用于各种生物传感领域。在这一领域，嵌入微流控技术的阻抗生物传感器的灵敏度，例如鞘流聚焦、介电泳聚焦和叉指电极阵列等，可以得到极大的提高。特别是，试剂消耗的减少和分析时间的缩短等特点，可以极大地提高这种生物传感器的分析能力。此外，微流控技术实现的自动化可以提高分析的可靠性和效率。通过使用成熟的微流控技术，可以将复杂的生物过程缩小并集成到单个微流控系统中（例如，芯片实验室或微全分析系统）。通过将阻抗生物传感器集成到其中，即使是非专业人员也可以轻松地开发和操作手持式和台式微流控系统。此外，阻抗谱提供了关于细胞大小、膜电容、细胞质电导率和细胞质介电常数的广泛信息，而无需进行荧光标记、磁性修饰或其他细胞处理。在这篇综述文章中，对基于微流控的阻抗生物传感器进行了全面的总结。本文的结构基于不同的衬底材料分类。此外，还讨论了基于微流控的阻抗生物传感器的发展趋势，以及未来可能遇到的困难和挑战。
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