Panessa-Warren B J, Tortora G T, Warren J B
Department of Allied Health Resources, State University of New York, Stony Brook 11794.
Ultramicroscopy. 1989 Mar;27(2):151-60. doi: 10.1016/0304-3991(89)90083-1.
This article describes a new method of viewing biological specimens by taking advantage of the absorptive characteristics of monochromatic X-rays above and below the absorption edge of a specific element. Bacterial endospores were imaged before and after treatment with an experimental vanadium-containing sporocide using monochromatic synchrotron radiation at the nitrogen absorption edge, and above and below the vanadium LIII absorption edge. This morphological study demonstrates a rapid, easy-to-use method of soft X-ray absorption edge imaging that can be used by the biologist to obtain morphological and elemental information that is not readily accessible using conventional microscopic and analytic techniques.
本文介绍了一种利用特定元素吸收边上下单色X射线的吸收特性来观察生物标本的新方法。使用氮吸收边以及钒LIII吸收边上下的单色同步辐射，对用一种实验性含钒杀孢子剂处理前后的细菌芽孢进行成像。这项形态学研究展示了一种快速、易用的软X射线吸收边成像方法，生物学家可利用该方法获取使用传统显微镜和分析技术不易获得的形态和元素信息。
