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[苏云金芽孢杆菌伴孢晶体的扫描隧道显微镜和电子显微镜观察]

[Scanning tunneling and electron microscopy of parasporal crystals in Bacillus thuringiensis].

作者信息

Solontsov I L, Iudina T G

出版信息

Mikrobiologiia. 1996 Mar-Apr;65(2):235-40.

PMID:8724755
Abstract

Comparison of the forms of parasporal inclusions in Bacillus thuringiensis subsp. kurstaki and B. thuringiensis subsp. tenebrionis under a scanning electron microscope showed that, in addition to bipyramidal and cuboid crystals, cells of the subspecies kurstaki are able to form crystals taking the shape of flat parallelopipeds typical of the subspecies tenebrionis. The inclusions in the subspecies tenebrionis vary in size and electron density. Examination of the structure of crystals in B. thuringiensis subsp. tenebrionis under a scanning tunneling microscope showed that delta-endotoxins in the crystals are assembled in globules 5.0 nm in diameter and 1.0 nm in height. The globules are connected by its bases and form chains. The subunits of tetragonal crystals in the subspecies tenebrionis are vertically oriented with respect to the chain axis, as was revealed by other methods for subunits of bipyramidal crystals of the subspecies kurstaki.

摘要

在扫描电子显微镜下对苏云金芽孢杆菌库尔斯塔克亚种和苏云金芽孢杆菌暗黑亚种伴胞晶体形态的比较表明,除了双金字塔形和长方体形晶体外,库尔斯塔克亚种的细胞还能够形成暗黑亚种特有的扁平平行六面体形状的晶体。暗黑亚种中的内含物在大小和电子密度上有所不同。在扫描隧道显微镜下对苏云金芽孢杆菌暗黑亚种晶体结构的检查表明,晶体中的δ-内毒素组装成直径5.0 nm、高1.0 nm的小球。这些小球通过其基部相连并形成链。暗黑亚种四方晶体的亚基相对于链轴垂直排列,这与库尔斯塔克亚种双金字塔形晶体亚基的其他方法所揭示的情况相同。

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