Yin Xin, Zhao Fu-Rong, Zhou Dong-Hui, Wei Ping, Chang Hui-Yun
College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150001, People's Republic of China.
Arch Virol. 2014 Nov;159(11):2877-82. doi: 10.1007/s00705-014-2119-y. Epub 2014 Jun 26.
From January to July 2012, we looked for evidence of subclinical A (H1N1) pdm09 and seasonal human influenza viruses infections in healthy dogs in China. Sera from a total of 1920 dogs were collected from Guangdong, Guangxi, Fujian and Jiangxi provinces. We also examined archived sera from 66 dogs and cats that were collected during 2008 from these provinces. Using hemagglutination inhibition (HI) and microneutralization (MN) assays, we found that only the dogs sampled in 2012 had elevated antibodies (≥ 1:32) against A(H1N1)pdm09 virus and seasonal human influenza viruses: Of the 1920 dog sera, 20.5 % (n = 393) had elevated antibodies against influenza A(H1N1) pdm09 by the HI assay, 1.1 % (n = 22), and 4.7 % (n = 91) of the 1920 dogs sera had elevated antibodies against human seasonal H1N1 influenza virus and human seasonal H3N2 influenza virus by the HI assay. Compared with dogs that were raised on farms, dogs that were raised as pets were more likely to have elevated antibodies against A(H1N1)pdm09 and seasonal human influenza viruses. Seropositivity was highest among pet dogs, which likely had more diverse and frequent exposures to humans than farm dogs. These findings will help us better understand which influenza A viruses are present in dogs and will contribute to the prevention and control of influenza A virus. Moreover, further in-depth study is necessary for us to understand what roles dogs play in the ecology of influenza A.
2012年1月至7月，我们在中国健康犬中寻找甲型H1N1pdm09流感病毒和季节性人流感病毒亚临床感染的证据。从广东、广西、福建和江西四省共采集了1920份犬血清样本。我们还检测了2008年从这些省份采集的66份犬猫存档血清样本。通过血凝抑制（HI）试验和微量中和（MN）试验，我们发现只有2012年采集样本的犬只对甲型H1N1pdm09流感病毒和季节性人流感病毒的抗体水平有所升高（≥1:32）：在1920份犬血清样本中，通过HI试验，20.5%（n = 393）的样本对甲型H1N1pdm09流感病毒的抗体水平升高；1920份犬血清样本中，分别有1.1%（n = 22）和4.7%（n = 91）的样本通过HI试验对季节性甲型H1N1流感病毒和季节性甲型H3N2流感病毒的抗体水平升高。与饲养在农场的犬相比，作为宠物饲养的犬更有可能对甲型H1N1pdm09流感病毒和季节性人流感病毒的抗体水平升高。宠物犬的血清阳性率最高，这可能是因为它们比农场犬接触人类的机会更多样、更频繁。这些发现将有助于我们更好地了解犬体内存在哪些甲型流感病毒，并有助于甲型流感病毒的防控。此外，有必要进一步深入研究，以了解犬在甲型流感病毒生态中的作用。
