在实验感染仓鼠的固定石蜡包埋组织中进行流感病毒抗原的免疫荧光染色。
Immunofluorescent staining of influenza virus antigen in fixed and paraffin-embedded tissue of experimentally infected hamsters.
作者信息
Lück P C, Helbig J H, Witzleb W
机构信息
Institute of Microbiology, Medical Academy Carl Gustav Carus, Dresden, GDR.
出版信息
Acta Histochem. 1989;85(1):47-50. doi: 10.1016/S0065-1281(89)80097-2.
Sections of formalin-fixed, paraffin-embedded tissue of experimentally influenza virus-infected hamsters were treated with 0.25% trypsin and tested for virus antigen by indirect immunofluorescent staining. The results were comparable to those obtained with aceton-fixed cryo-microtome sections. As far as we know, this is the first description of influenza virus demonstration in formalin-fixed, paraffin-embedded tissue after reactivation by trypsin-treatment. This technique may be useful for influenza virus detection in human autopsy cases. It allows an etiological diagnosis even when fresh tissue for cryocut sections or virus cultivation is not available.
将实验性感染流感病毒的仓鼠的福尔马林固定、石蜡包埋组织切片用0.25%胰蛋白酶处理，并用间接免疫荧光染色检测病毒抗原。结果与用丙酮固定的冷冻切片获得的结果相当。据我们所知，这是首次描述经胰蛋白酶处理后重新激活的福尔马林固定、石蜡包埋组织中的流感病毒。该技术可能对人类尸检病例中的流感病毒检测有用。即使没有用于冷冻切片或病毒培养的新鲜组织，它也能进行病因诊断。
Zotero 插件