Lemee Véronique, Leoz Marie, Etienne Manuel, De Oliveira Fabienne, Plantier Jean-Christophe
Laboratoire de Virologie Associé au Centre National de Référence du VIH, Hôpital Charles Nicolle, CHU de Rouen, Rouen, France GRAM, Equipe d'Accueil 2656, Faculté de Médecine-Pharmacie, Institut de Recherche et d'Innovation en Biomédecine, Université de Rouen, Rouen, France.
Laboratoire de Virologie Associé au Centre National de Référence du VIH, Hôpital Charles Nicolle, CHU de Rouen, Rouen, France GRAM, Equipe d'Accueil 2656, Faculté de Médecine-Pharmacie, Institut de Recherche et d'Innovation en Biomédecine, Université de Rouen, Rouen, France COREVIH Haute-Normandie, Hôpital Charles Nicolle, CHU de Rouen, Rouen, France.
J Clin Microbiol. 2014 Sep;52(9):3277-9. doi: 10.1128/JCM.01089-14. Epub 2014 Jun 25.
Screening for HIV infection has improved since the first immunoassays. Today, diagnosis of HIV infection can be performed with fourth-generation tests that track both the patient's antibodies and HIV antigen. The objective of this study was to evaluate the clinical sensitivity and specificity of the new DiaSorin Liaison XL Murex HIV Ab/Ag assay compared to another fourth-generation assay, the Abbott Architect HIV Ag/Ab Combo kit. This work was performed on a large panel of 900 samples, including negative samples (n = 493) and HIV-positive (n = 407) representatives of HIV-1 group M subtypes and circulating recombinant forms (CRFs), HIV-1 group O, and HIV-2 variants. The results highlight the high specificity (98.9%) and sensitivity (100%) of this new fourth-generation assay, which are consistent with its use for the screening and diagnosis of HIV infections with the current circulating strains.
自首次免疫测定以来，HIV感染筛查已有改进。如今，可通过追踪患者抗体和HIV抗原的第四代检测方法来诊断HIV感染。本研究的目的是评估新型DiaSorin Liaison XL Murex HIV Ab/Ag检测法相对于另一种第四代检测法Abbott Architect HIV Ag/Ab Combo试剂盒的临床敏感性和特异性。这项工作是在900个样本的大样本组上进行的，包括阴性样本（n = 493）以及HIV-1 M组亚型、循环重组型（CRF）、HIV-1 O组和HIV-2变异株的HIV阳性样本（n = 407）。结果表明，这种新型第四代检测法具有高特异性（98.9%）和敏感性（100%），这与其用于筛查和诊断当前流行毒株的HIV感染相符。
