唾液腺中的CD4+CXCR5+滤泡辅助性T细胞促进原发性干燥综合征患者B细胞成熟。
CD4+CXCR5+ follicular helper T cells in salivary gland promote B cells maturation in patients with primary Sjogren's syndrome.
作者信息
Jin Li, Yu Daliang, Li Xiaomei, Yu Ning, Li Xiangpei, Wang Yuanmin, Wang Yiping
机构信息
Department of Rheumatology and Immunology, Anhui Provincial Hospital Affiliated to Anhui Medical University Hefei, Anhui 230001, China.
Centre for Kidney Research, Children's Hospital at Westmead, Westmead, The University of Sydney NSW 2145, Australia.
出版信息
Int J Clin Exp Pathol. 2014 Apr 15;7(5):1988-96. eCollection 2014.
OBJECTIVE
To examine amount of CD4+CXCR5+Tfh cells and B cells subsets in salivary gland and peripheral blood from patients with primary Sjogren's syndrome (pSS) and to analyze whether the frequency of CD4+CXCR5+Tfh cells is associated with pSS pathologic process.
METHODS
The percentages of CD4+CXCR5+Tfh cells and B cell subsets were examined by flow cytometry. B-lymphocyte chemoattraetant (BLC; also called CXCL13), IL-21, IL-6 from the serum of pSS patients was assessed by polymerase chain reaction-enzyme-linked immunosorbent assay (ELISA).
RESULTS
The percentages of CD4+CXCR5+Tfh cells in peripheral blood were increased in pSS patients, but decreased after treatment with glucocorticoid and/or immunosuppressive drugs. Abnormal B cell subsets appeared in salivary and peripheral blood of pSS patients. The frequency of salivary CD4+CXCR5+Tfh cells was positively correlated with CD19+CD27+ memory B cells and CD19+CD27high plasma cells. Also increase of salivary CD19+CD27high plasma cells was positively associated with serum ANA titer of pSS patients.
CONCLUSIONS
CD4+CXCR5+Tfh cells are significantly increased in salivary and peripheral blood in pSS patients with aberrant CD19+CD27+ memory B cells and CD19+CD27high plasma cells, suggesting that CD4+CXCR5+Tfh cells may contribute to the pathogenesis of pSS by promoting the maturation of B cells.
目的
检测原发性干燥综合征（pSS）患者唾液腺和外周血中CD4⁺CXCR5⁺Tfh细胞及B细胞亚群的数量，并分析CD4⁺CXCR5⁺Tfh细胞频率是否与pSS病理进程相关。
方法
采用流式细胞术检测CD4⁺CXCR5⁺Tfh细胞及B细胞亚群的百分比。采用聚合酶链反应-酶联免疫吸附测定（ELISA）法检测pSS患者血清中的B淋巴细胞趋化因子（BLC；亦称为CXCL13）、IL-21、IL-6。
结果
pSS患者外周血中CD4⁺CXCR5⁺Tfh细胞百分比升高，但经糖皮质激素和/或免疫抑制药物治疗后降低。pSS患者唾液和外周血中出现异常B细胞亚群。唾液中CD4⁺CXCR5⁺Tfh细胞频率与CD19⁺CD27⁺记忆B细胞及CD19⁺CD27high浆细胞呈正相关。唾液中CD19⁺CD27high浆细胞增加也与pSS患者血清ANA滴度呈正相关。
结论
pSS患者唾液和外周血中CD4⁺CXCR5⁺Tfh细胞显著增加，伴有异常的CD19⁺CD27⁺记忆B细胞及CD19⁺CD27high浆细胞，提示CD4⁺CXCR5⁺Tfh细胞可能通过促进B细胞成熟参与pSS的发病机制。
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