Villegas-Ruíz Vanessa, Salcedo Mauricio, Zentella-Dehesa Alejandro, de Oca Edén V Montes, Román-Basaure Edgar, Mantilla-Morales Alejandra, Dávila-Borja Víctor M, Juárez-Méndez Sergio
Genomic Oncology Laboratory, Medical Research Unit in Oncologic Diseases, Oncology Hospital, National Medical Center Siglo XXI, IMSS México, D.F., México.
Department of Medical Genomics and Environmental Toxicology, Biomedical Research Institute, UNAM México, D.F., México ; Biochemistry Unit, National Institute of Medical Sciences and Nutrition "Salvador Zubirán" México, D.F., México.
Int J Clin Exp Pathol. 2014 Apr 15;7(5):2256-64. eCollection 2014.
Cervical cancer is the second malignancy in Mexico, little is known about the prognostic factors associated with this disease. Several cellular components are important in their transformation and progression. Alternative mRNA splice is an important mechanism for generating protein diversity, nevertheless, in cancer unknown mRNA diversity is expressed. Hyaluronan-mediated motility receptor (HMMR, RHAMM, CD168) is a family member of proteins, hyaluronan acid dependent, and has been associated with different malignant processes such as: angiogenesis, cell invasiveness, proliferation, metastasis and poor outcome in some tumors. In the present study we identified expression of HMMR in cervical cancer by means of RT-PCR and sequencing. Our results indicate co-expression of two HMMR variants in all samples, and one case expressed three alternative HMMR splice transcripts. These results showed the heterogeneity of mRNA transcripts of HMMR that could express in cancer and the expression of HMMR could be marker of malignancy in CC.
宫颈癌是墨西哥第二大恶性肿瘤，人们对与该疾病相关的预后因素知之甚少。几种细胞成分在其转化和进展过程中很重要。可变mRNA剪接是产生蛋白质多样性的重要机制，然而，在癌症中未知的mRNA多样性会表达出来。透明质酸介导的运动受体（HMMR，RHAMM，CD168）是一类依赖透明质酸的蛋白质家族成员，并且与不同的恶性过程相关，如：血管生成、细胞侵袭、增殖、转移以及某些肿瘤的不良预后。在本研究中，我们通过逆转录聚合酶链反应（RT-PCR）和测序鉴定了HMMR在宫颈癌中的表达。我们的结果表明，所有样本中均共表达两种HMMR变体，并且有一个病例表达了三种可变的HMMR剪接转录本。这些结果显示了HMMR在癌症中可能表达的mRNA转录本的异质性，并且HMMR的表达可能是宫颈癌恶性程度的标志物。
