Demirtas Sinan, Karahan Oguz, Yazici Suleyman, Guclu Orkut, Caliskan Ahmet, Tezcan Orhan, Yavuz Celal
Medical School of Dicle University, Department of Cardiovascular Surgery, Diyarbakir, Turkey.
Cardiovasc J Afr. 2014 Sep-Oct;25(5):200-3. doi: 10.5830/CVJA-2014-033. Epub 2014 Jun 26.
Mesenteric arteries release C-type natriuretic peptide (CNP), which hyperpolarises vascular smooth muscle. We measured the levels of this peptide after inducing mesenteric ischaemia over a series of time intervals, so as to determine its predictive value in demonstrating the severity of ischaemia in a rat model.
A total of 32 rats were allocated to four groups containing eight rats each. Basal CNP reference levels were measured in the control group, which was not exposed to any intervention. In groups I, II and III, mesenteric ischaemia was induced over three, six and nine hours, respectively, and plasma CNP levels were measured afterwards. Mesenteric ischaemia was induced by clamping the superior mesenteric artery.
In comparison with the controls (2.38 ± 0.18 pg/ml), CNP levels were relatively lower in group I (2.54 ± 0.42 pg/ml). However, significant increases in plasma CNP levels were observed over longer periods of ischaemia in group II, at 5.23 ± 0.22 pg/ml, and in group III, at 6.19 ± 0.67 pg/ml (p < 0.05). A significant direct relationship was determined between plasma CNP levels and prolonged intervals of mesenteric ischaemia (R = 0.56, p < 0.001).
Measuring plasma CNP levels in patients with acute mesenteric ischaemia may be beneficial in estimating the time period over which the ischaemic injury has occurred.
肠系膜动脉释放C型利钠肽（CNP），它可使血管平滑肌超极化。我们在一系列时间间隔内诱导肠系膜缺血后测量了这种肽的水平，以确定其在大鼠模型中显示缺血严重程度的预测价值。
总共32只大鼠被分为四组，每组8只。在未接受任何干预的对照组中测量基础CNP参考水平。在第一、二、三组中，分别诱导3、6和9小时的肠系膜缺血，之后测量血浆CNP水平。通过夹闭肠系膜上动脉诱导肠系膜缺血。
与对照组（2.38±0.18 pg/ml）相比，第一组的CNP水平相对较低（2.54±0.42 pg/ml）。然而，在第二组中，缺血时间较长时血浆CNP水平显著升高，为5.23±0.22 pg/ml，第三组为6.19±0.67 pg/ml（p<0.05）。血浆CNP水平与肠系膜缺血的延长时间之间存在显著的直接关系（R=0.56，p<0.001）。
测量急性肠系膜缺血患者的血浆CNP水平可能有助于估计缺血损伤发生的时间段。
