Xiu Meng, Pan Luqing, Jin Qian
The Key Laboratory of Mariculture, Fisheries College, Ministry of Education, Ocean University of China, Yushan Road 5, Qingdao 266003, PR China.
The Key Laboratory of Mariculture, Fisheries College, Ministry of Education, Ocean University of China, Yushan Road 5, Qingdao 266003, PR China.
Ecotoxicol Environ Saf. 2014 Sep;107:103-10. doi: 10.1016/j.ecoenv.2014.05.016. Epub 2014 Jun 24.
The study is aimed at investigating the bioaccumulation and oxidative damage of juvenile scallops (Chlamys farreri) exposed to three selected PAHs: benzo[a]pyrene (BaP), benzo[b]fluoranthene (BbF) and chrysene (CHR). For this purpose, a study was performed on juvenile scallops exposed to BaP (0.01, 0.2 and 4μg/L), BbF (0.02, 0.2 and 2μg/L) and CHR (0.2, 0.8 and 3.2μg/L) for 21 days. Accumulations of these three PAHs in soft parts of scallops, except the 0.01μg/L BaP group and the 0.02μg/L BbF group, showed obvious time and dose dependence, and CHR accumulation was higher when compared to BaP and BbF. Oxidative damage indicators, including lipid peroxidation (LPO), protein carbonyl (PC) and DNA strand breaks, were also measured in soft parts to assess effects of the selected PAHs. The results showed that the LPO levels, PC contents and DNA damage were induced significantly (P<0.05 or P<0.01), except in the low level groups of BaP and BbF, and different trends were detected with time of exposure. According to the correlation analysis results, PC content in soft parts showed a good correlation with the target contaminant and seemed to be proposed as a potential early indicator of BaP, BbF and CHR. In addition the sequence of toxicity is BaP>BbF>CHR, judging by the level of induction of oxidative damage at 0.2μg/L levels. The results of this research are expected to contribute to the establishment of a good biochemical index of exposure to PAHs in laboratory experiments, which can be further useful in field studies.
本研究旨在调查暴露于三种选定多环芳烃（PAHs）的栉孔扇贝幼贝（Chlamys farreri）的生物累积和氧化损伤情况，这三种多环芳烃分别为苯并[a]芘（BaP）、苯并[b]荧蒽（BbF）和屈（CHR）。为此，对暴露于BaP（0.01、0.2和4μg/L）、BbF（0.02、0.2和2μg/L）和CHR（0.2、0.8和3.2μg/L）21天的栉孔扇贝幼贝进行了研究。除0.01μg/L BaP组和0.02μg/L BbF组外，这三种多环芳烃在扇贝软组织中的累积呈现出明显的时间和剂量依赖性，并且CHR的累积量高于BaP和BbF。还测量了软组织中的氧化损伤指标，包括脂质过氧化（LPO）、蛋白质羰基（PC）和DNA链断裂，以评估所选多环芳烃的影响。结果表明，除了BaP和BbF的低剂量组外，LPO水平、PC含量和DNA损伤均显著诱导（P<0.05或P<0.01），并且随着暴露时间的不同呈现出不同趋势。根据相关性分析结果，软组织中的PC含量与目标污染物显示出良好的相关性，似乎可作为BaP、BbF和CHR的潜在早期指标。此外，以0.2μg/L水平的氧化损伤诱导程度判断，毒性顺序为BaP>BbF>CHR。本研究结果有望有助于在实验室实验中建立良好的多环芳烃暴露生化指标，这在野外研究中可能会更有用。
