Jing-Jing Miao, Lu-qing Pan, Jing Liu, Lin Zhang
The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao, China.
Mar Environ Res. 2009 Feb;67(1):47-52. doi: 10.1016/j.marenvres.2008.10.006. Epub 2008 Nov 7.
To investigate the biological impact of polycyclic aromatic hydrocarbons (PAHs) on reproductive system, scallops Chlamys farreri were continuously exposed to benzo[a]pyrene (BaP) (0.5, 3, 10microgL(-1)) during 15 days. DNA damage and histological alterations were examined in female gonad. DNA strand break levels significantly increased after 12h exposure, and remained high and significantly different from control values until the end of the exposure. In the ovaries of the scallops exposed to 10microgL(-1) BaP for ten days, histological analysis showed that the cytoplasts of the oocytes in the outer layer of the ovaries became sparse, and the nuclear membranes were obscure. Damaging effects on ovaries and oocytes were more severe after 15 days exposure. Degenerating oocytes increased and the connective tissue of the ovary envelops became loose. Electron microscopic examination revealed ultrastructural aspects of degenerating oocyte and degenerated oocyte after 15 days exposure.
为研究多环芳烃（PAHs）对生殖系统的生物学影响，将栉孔扇贝连续15天暴露于苯并[a]芘（BaP）（0.5、3、10μg L-1）中。检测了雌性性腺中的DNA损伤和组织学变化。暴露12小时后，DNA链断裂水平显著增加，并在暴露结束前一直保持在较高水平，且与对照值有显著差异。在暴露于10μg L-1 BaP十天的扇贝卵巢中，组织学分析表明，卵巢外层卵母细胞的细胞质变得稀疏，核膜模糊。暴露15天后，对卵巢和卵母细胞的损伤作用更严重。退化的卵母细胞增加，卵巢包膜的结缔组织变得疏松。电子显微镜检查揭示了暴露15天后退化卵母细胞和变性卵母细胞的超微结构特征。
