采用绿液与乙醇混合有机溶剂预处理提高糠醛渣的酶水解性能。
Improvement of the enzymatic hydrolysis of furfural residues by pretreatment with combined green liquor and ethanol organosolv.
机构信息
Department of Chemistry and Chemical Engineering, Beijing Forestry University, Beijing, China.
GuangXi Key Laboratory of Chemistry and Engineering of Forest Products, Nanning 530006, China.
出版信息
Bioresour Technol. 2014 Sep;167:46-52. doi: 10.1016/j.biortech.2014.05.111. Epub 2014 Jun 5.
Furfural residues (FRs) were pretreated with ethanol and a green liquor (GL) catalyst to produce fermentable sugar. Anthraquinone (AQ) was used as an auxiliary reagent to improve delignification and reduce cellulose decomposition. The results showed that 42.7% of lignin was removed and 96.5% of cellulose was recovered from substrates pretreated with 1.0 mL GL/g of dry substrate and 0.4% (w/w) AQ at 140°C for 1h. Compared with raw material, ethanol-GL pretreatment of FRs increased the glucose yield from 69.0% to 85.9% after 96 h hydrolysis with 18 FPU/g-cellulose for cellulase, 27 CBU/g-cellulose for β-glucosidase. The Brauner-Emmett-Teller surface area was reduced during pretreatment, which did not inhibit the enzymatic hydrolysis. Owing to the reduced surface area, the unproductive binding of cellulase to lignin was decreased, thus improving the enzymatic hydrolysis. The degree of polymerization of cellulose from FRs was too low to be a key factor for improving enzymatic hydrolysis.
糠醛渣（FRs）经乙醇和绿液（GL）催化剂预处理后可产生可发酵糖。蒽醌（AQ）被用作辅助试剂，以提高脱木质素效率并减少纤维素分解。结果表明，在 140°C 下，用 1.0 mL GL/g 干底物和 0.4%（w/w）AQ 预处理 1h 后，从底物中去除了 42.7%的木质素，回收了 96.5%的纤维素。与原料相比，在纤维素酶 18 FPU/g-纤维素、β-葡萄糖苷酶 27 CBU/g-纤维素作用下，96 h 水解后，乙醇-GL 预处理 FRs 使葡萄糖得率从 69.0%提高到 85.9%。预处理过程中，BET 表面积减小，但并未抑制酶解。由于表面积减小，纤维素酶与木质素的非生产性结合减少，从而提高了酶解效率。FRs 中的纤维素聚合度太低，不是提高酶解效率的关键因素。
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