Department of Chemistry and Chemical Engineering, Beijing Forestry University, Beijing 100083, China.
Department of Chemistry and Chemical Engineering, Beijing Forestry University, Beijing 100083, China; GuangXi Key Laboratory of Chemistry and Engineering of Forest Products, Nanning 530006, China.
Bioresour Technol. 2013 Nov;147:29-36. doi: 10.1016/j.biortech.2013.08.013. Epub 2013 Aug 9.
A potential commercial pretreatment for furfural residues (FRs) was investigated by using a combination of green liquor and hydrogen peroxide (GL-H2O2). The results showed that 56.2% of lignin removal was achieved when the sample was treated with 0.6 g H2O2/g-DS (dry substrate) and 6 mL GL/g-DS at 80 °C for 3 h. After 96 h hydrolysis with 18 FPU/g-cellulose for cellulase, 27 CBU/g-cellulose for β-glucosidase, the glucose yield increased from 71.2% to 83.6%. Ethylenediaminetetraacetic acid was used to reduce the degradation of H2O2, the glucose yield increased to 90.4% after the addition of 1% (w/w). The untreated FRs could bind more easily to cellulase than pretreated FRs could. The structural changes on the surface of sample were characterized by X-ray photoelectron spectroscopy. The results indicated that the surface lignin could be effectively removed during pretreatment, thereby decreasing the enzyme-lignin binding activity. Moreover, the carbonyl from lignin plays an important role in cellulase binding.
研究了一种用绿液和过氧化氢（GL-H2O2）联合处理糠醛渣（FRs）的潜在商业预处理方法。结果表明，当样品用 0.6 g H2O2/g-DS（干底物）和 6 mL GL/g-DS 在 80°C 下处理 3 h 时，木质素去除率达到 56.2%。用 18 FPU/g-纤维素的纤维素酶水解 96 h 后，用 27 CBU/g-纤维素的β-葡萄糖苷酶水解，葡萄糖得率从 71.2%增加到 83.6%。用乙二胺四乙酸（EDTA）减少 H2O2 的降解，添加 1%（w/w）的 EDTA 后，葡萄糖得率增加到 90.4%。未经处理的 FRs 比预处理的 FRs 更容易与纤维素酶结合。通过 X 射线光电子能谱对样品表面的结构变化进行了表征。结果表明，预处理过程中可以有效地去除表面木质素，从而降低酶-木质素结合活性。此外，木质素中的羰基在纤维素酶结合中起着重要作用。
