用绿液和双氧水联合预处理提高糠醛渣的酶水解效率。
Improvement of the enzymatic hydrolysis of furfural residues by pretreatment with combined green liquor and hydrogen peroxide.
机构信息
Department of Chemistry and Chemical Engineering, Beijing Forestry University, Beijing 100083, China.
Department of Chemistry and Chemical Engineering, Beijing Forestry University, Beijing 100083, China; GuangXi Key Laboratory of Chemistry and Engineering of Forest Products, Nanning 530006, China.
出版信息
Bioresour Technol. 2013 Nov;147:29-36. doi: 10.1016/j.biortech.2013.08.013. Epub 2013 Aug 9.
A potential commercial pretreatment for furfural residues (FRs) was investigated by using a combination of green liquor and hydrogen peroxide (GL-H2O2). The results showed that 56.2% of lignin removal was achieved when the sample was treated with 0.6 g H2O2/g-DS (dry substrate) and 6 mL GL/g-DS at 80 °C for 3 h. After 96 h hydrolysis with 18 FPU/g-cellulose for cellulase, 27 CBU/g-cellulose for β-glucosidase, the glucose yield increased from 71.2% to 83.6%. Ethylenediaminetetraacetic acid was used to reduce the degradation of H2O2, the glucose yield increased to 90.4% after the addition of 1% (w/w). The untreated FRs could bind more easily to cellulase than pretreated FRs could. The structural changes on the surface of sample were characterized by X-ray photoelectron spectroscopy. The results indicated that the surface lignin could be effectively removed during pretreatment, thereby decreasing the enzyme-lignin binding activity. Moreover, the carbonyl from lignin plays an important role in cellulase binding.
研究了一种用绿液和过氧化氢（GL-H2O2）联合处理糠醛渣（FRs）的潜在商业预处理方法。结果表明，当样品用 0.6 g H2O2/g-DS（干底物）和 6 mL GL/g-DS 在 80°C 下处理 3 h 时，木质素去除率达到 56.2%。用 18 FPU/g-纤维素的纤维素酶水解 96 h 后，用 27 CBU/g-纤维素的β-葡萄糖苷酶水解，葡萄糖得率从 71.2%增加到 83.6%。用乙二胺四乙酸（EDTA）减少 H2O2 的降解，添加 1%（w/w）的 EDTA 后，葡萄糖得率增加到 90.4%。未经处理的 FRs 比预处理的 FRs 更容易与纤维素酶结合。通过 X 射线光电子能谱对样品表面的结构变化进行了表征。结果表明，预处理过程中可以有效地去除表面木质素，从而降低酶-木质素结合活性。此外，木质素中的羰基在纤维素酶结合中起着重要作用。
Zotero 插件