京尼平交联丝素蛋白/壳聚糖控释微球的制备与表征
[Preparation and characterization of genipin-crosslinked silk fibroin/chitosan controlled-release microspheres].
作者信息
Ye Manwen, Zeng Shuguang, Gao Wenfeng, Rong Mingdeng, Guo Zehong, Shi Yong, Gan Huiyong
机构信息
Guangdong Provincial Stomatological Hospital Affiliated to Southern Medical University, Guangzhou 510220, China.E-mail:
出版信息
Nan Fang Yi Ke Da Xue Xue Bao. 2014 Jun;34(6):875-9.
OBJECTIVE
To investigate the property of genipin-crosslinked silk fibroin(SF)/chitosan(CS) microspheres for slow releasing of bovine serum albumin (BSA).
METHODS
BSA-loaded genipin-crosslinked SF/CS microspheres were prepared by emulsion cross-linking technique. The micropheres were observed for surface morphology and size distribution under scanning electron microscope (SEM), and X-ray diffractometry (XRD) and fourier transform infrared spectroscopy (FTIR) were used to analyze their structural characteristics. BCA method was used for determining the drug entrapment, loading rate and cumulative drug release in 21 days.
RESULT
The microspheres were spherical and showed a smooth surface with an average diameter of 7.84∓0.97 µm. The drug entrapment efficiency of the microspheres was (50.16∓4.32)% with a drug loading ratio of (1.25∓0.11)% and a cumulative release of the total drug of (75.2∓2.53)% in 21 days.
CONCLUSION
Genipin-crosslinked SF/CS microspheres have a high drug entrapment efficiency and possess good capacity of sustained drug release.
目的
研究京尼平交联丝素蛋白（SF）/壳聚糖（CS）微球对牛血清白蛋白（BSA）的缓释性能。
方法
采用乳化交联技术制备载有BSA的京尼平交联SF/CS微球。在扫描电子显微镜（SEM）下观察微球的表面形态和粒径分布，并用X射线衍射仪（XRD）和傅里叶变换红外光谱仪（FTIR）分析其结构特征。采用BCA法测定药物包封率、载药率及21天内的药物累积释放量。
结果
微球呈球形，表面光滑，平均直径为7.84±0.97μm。微球的药物包封率为（50.16±4.32）%，载药率为（1.25±0.11）%，21天内药物累积释放量为（75.2±2.53）%。
结论
京尼平交联SF/CS微球具有较高的药物包封率和良好的缓释性能。
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