LEPABE - Laboratory for Process Engineering, Environment, Biotechnology & Energy, Faculty of Engineering of Porto, Department of Chemical Engineering, University of Porto, Porto, Portugal.
Universidade Católica Portuguesa, CBQF - Centro de Biotecnologia e Química Fina - Laboratório Associado, Escola Superior de Biotecnologia, Rua Diogo Botelho 1327, 4169-005 Porto, Portugal.
IET Nanobiotechnol. 2020 Oct;14(8):645-653. doi: 10.1049/iet-nbt.2020.0058.
Cell-seeded microcarriers (MCs) are currently one of the most promising topics in biotechnology. These systems are supportive structures for cell growth and expansion that allow efficient nutrient and gas transfer between the media and the attached cells. Silk proteins have been increasingly used for this purpose in the past few years due to their biocompatibility, biodegradability and non-toxicity. To date, several silk fibroin spherical MCs in combination with alginate, gelatin and calcium phosphates have been reported with very interesting outcomes. In addition, other silk-based three-dimensional structures such as microparticles with chitosan and collagen, as well as organoids, have been increasingly studied. In this study, the physicochemical and biological properties of these biomaterials, as well as the recent methodologies for their processing and for cell culture, are discussed. The potential biomedical applications are also addressed. In addition, an analysis of the future perspectives is presented, where the potential of innovative silk-based MCs processing technologies is highlighted.
细胞接种微载体（MCs）是目前生物技术中最有前途的课题之一。这些系统是细胞生长和扩增的支撑结构，允许介质和附着细胞之间高效地进行营养物质和气体传递。在过去几年中，由于丝蛋白具有生物相容性、可生物降解性和无毒性，因此越来越多地被用于此目的。迄今为止，已经报道了几种丝素球形 MCs 与藻酸盐、明胶和磷酸钙结合，具有非常有趣的结果。此外，其他基于丝的三维结构，如带有壳聚糖和胶原蛋白的微颗粒以及类器官，也越来越受到研究。在这项研究中，讨论了这些生物材料的物理化学和生物学特性，以及最近用于其加工和细胞培养的方法。还讨论了它们的潜在生物医学应用。此外，还对未来的前景进行了分析，强调了创新的丝基 MCs 加工技术的潜力。
