Institute of Stem Cell and Regenerative Medicine, Medical College, Xiamen University, Xiamen, 361102, China.
Nanoscale. 2014 Aug 7;6(15):9025-33. doi: 10.1039/c4nr01004c.
Stem cell based therapies offer significant potential in the field of regenerative medicine. The development of superparamagnetic iron oxide (SPIO) nanoparticle labeling and magnetic resonance imaging (MRI) have been increasingly used to track the transplanted cells, enabling in vivo determination of cell fate. However, the impact of SPIO-labeling on the cell phenotype and differentiation capacity of embryonic stem cells (ESCs) remains unclear. In this study, we wrapped SPIO nanoparticles with stearic acid grafted PEI600, termed as Stearic-LWPEI-SPIO, to generate efficient and non-toxic ESC labeling tools. Our results showed that efficient labeling of ESCs at an optimized low dosage of Stearic-LWPEI-SPIO nanoparticles did not alter the differentiation and self-renewal properties of ESCs. The localization of the transplanted ESCs observed by MRI correlated well with histological studies. These findings demonstrate that Stearic-LWPEI-SPIO nanoparticles have potential to be clinically translatable MRI probes and may enable non-invasive in vivo tracking of ESCs in experimental and clinical settings during cell-based therapies.
基于干细胞的疗法在再生医学领域具有重要的应用潜力。超顺磁性氧化铁 (SPIO) 纳米颗粒标记和磁共振成像 (MRI) 的发展越来越多地用于跟踪移植细胞，从而能够在体内确定细胞命运。然而，SPIO 标记对胚胎干细胞 (ESCs) 的细胞表型和分化能力的影响尚不清楚。在这项研究中，我们用接枝有聚醚酰亚胺 600 的硬脂酸包裹 SPIO 纳米颗粒，称为 Stearic-LWPEI-SPIO，以生成高效且无毒的 ESC 标记工具。我们的结果表明，在优化的低剂量下，Stearic-LWPEI-SPIO 纳米颗粒可以有效地标记 ESC，而不会改变 ESC 的分化和自我更新特性。通过 MRI 观察到的移植 ESC 的定位与组织学研究很好地吻合。这些发现表明，Stearic-LWPEI-SPIO 纳米颗粒有可能成为临床可转化的 MRI 探针，并可能能够在实验和临床环境中的细胞治疗期间实现对 ESC 的非侵入性体内跟踪。
