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对25个墨西哥普通豆(菜豆属菜豆)品种的叶酚作为抗氧化剂和品种标记物的评估。

Evaluation of foliar phenols of 25 Mexican varieties of common bean (Phaseolus vulgaris L.) as antioxidants and varietal markers.

作者信息

Reyes-Martínez Alfonso, Almaraz-Abarca Norma, Gallardo-Velázquez Tzayhri, González-Elizondo María Del Socorro, Herrera-Arrieta Yolanda, Pajarito-Ravelero Arnulfo, Alanís-Bañuelos Ruth Elizabeth, Torres-Morán Martha Isabel

机构信息

a Centro Interdisciplinario de Investigación para el Desarrollo Integral Regional, Instituto Politécnico Nacional , 34220 Durango , Mexico.

出版信息

Nat Prod Res. 2014;28(23):2158-62. doi: 10.1080/14786419.2014.930855. Epub 2014 Jun 27.

Abstract

The antioxidant properties and the foliar phenol composition of 25 Mexican varieties of Phaseolus vulgaris L. (common bean) were evaluated. Phaseolus coccineus was analysed with comparative aims. The high performance liquid chromatography with photodiode array detection analysis revealed 27 phenolics in the leaves of P. vulgaris (13 quercetin-3-O-glycosides, 8 kaempferol-3-O-glycosides, 2 myricetin glycosides and 4 phenolic acids) and 5 in P. coccineus (2 kaempferol-3-O-glycoside, 2 apigenin-7-O-glycoside and 1 luteolin-7-O-glycoside). All extracts showed high levels of phenols and flavonoids (0.964-5.601 mg g⁻¹ dry tissue, and 0.287-1.418 mg g⁻¹ dry tissue, respectively) and relevant antioxidant properties, suggesting that the leaves of the varieties of P. vulgaris are a significant source of natural antioxidants. The foliar phenol profiles were species-specific and, besides, the qualitative variation allowed discriminating among varieties of P. vulgaris. These profiles can represent an important varietal authenticity proof.

摘要

对25个墨西哥菜豆品种的抗氧化特性和叶片酚类成分进行了评估。对多花菜豆进行了对比分析。高效液相色谱-光电二极管阵列检测分析显示,菜豆叶片中有27种酚类物质(13种槲皮素-3-O-糖苷、8种山奈酚-3-O-糖苷、2种杨梅素糖苷和4种酚酸),多花菜豆叶片中有5种(2种山奈酚-3-O-糖苷、2种芹菜素-7-O-糖苷和1种木犀草素-7-O-糖苷)。所有提取物均显示出高水平的酚类和黄酮类物质(分别为0.964 - 5.601毫克/克干组织和0.287 - 1.418毫克/克干组织)以及相关的抗氧化特性,这表明菜豆品种的叶片是天然抗氧化剂的重要来源。叶片酚类谱具有物种特异性,此外,定性差异能够区分菜豆的不同品种。这些谱可以作为重要的品种真实性证据。

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