Department of Food Science, University of Manitoba, Winnipeg, Manitoba R3T, Canada.
Department of Food Science, University of Manitoba, Winnipeg, Manitoba R3T, Canada.
Food Chem. 2019 Jan 30;272:26-32. doi: 10.1016/j.foodchem.2018.07.220. Epub 2018 Aug 1.
Seed coats of coloured dry beans contain biologically active compounds. Flavonol glycosides were identified from acetone extracts of seed coats of black beans, pinto beans, and red kidney beans and evaluated for antioxidant activity. High Performance Liquid Chromatography - Mass Spectrometry/Mass Spectrometry (HPLC-MS/MS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging capacity were used to measure flavonols and antioxidant capacity, respectively. The main flavonol glycosides in black beans were the 3-O-glycosides of kaempferol, quercetin and myricetin; pinto beans contained kaempferol 3-O-glycosides, while red kidney beans contained quercetin 3-O-glycoside and quercetin 3-O-rutinoside (rutin). A flavonol triglycoside was identified in dark red kidney bean. Despite differences in total flavonol content, extracts from Eclipse, (black bean) and Windbreaker (pinto bean) had the significantly higher antioxidant activities than other bean seed coats. Results suggest seed coats of Windbreaker and Eclipse may have potential as functional food ingredients, though benefits may not be simply due to flavonols.
有色干豆的种皮含有具有生物活性的化合物。从黑芸豆、斑豆和红芸豆的种皮的丙酮提取物中鉴定出了类黄酮糖苷，并评估了它们的抗氧化活性。高效液相色谱-质谱/质谱联用（HPLC-MS/MS）和 2,2-二苯基-1-苦基肼基（DPPH）自由基清除能力分别用于测量类黄酮和抗氧化能力。黑芸豆中的主要类黄酮糖苷为山奈酚、槲皮素和杨梅素的 3-O-糖苷；斑豆含有山奈酚 3-O-糖苷，而红芸豆含有槲皮素 3-O-糖苷和槲皮素 3-O-芸香糖苷（芦丁）。在深红豆中鉴定出了一种类黄酮三糖苷。尽管总类黄酮含量存在差异，但 Eclipse（黑芸豆）和 Windbreaker（斑豆）的提取物的抗氧化活性明显高于其他豆种皮的提取物。结果表明，Windbreaker 和 Eclipse 的种皮可能具有作为功能性食品成分的潜力，尽管其益处可能不仅仅归因于类黄酮。
