利用弱物理相互作用构建互锁 DNA 环。
Engineering interlocking DNA rings with weak physical interactions.
机构信息
1] Department of Biochemistry and Biomedical Sciences, Michael G. DeGroote Institute of Infectious Disease Research, McMaster University, 1280 Main Street West, Hamilton Ontario, Canada L8S 4K1 [2] Department of Chemistry and Chemical Biology, 1280 Main Street West, Hamilton Ontario, Canada L8S 4K1 [3] Cancer Metastasis Alert and Prevention Center, College of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou 350002, China [4].
1] Department of Biochemistry and Biomedical Sciences, Michael G. DeGroote Institute of Infectious Disease Research, McMaster University, 1280 Main Street West, Hamilton Ontario, Canada L8S 4K1 [2] Department of Chemistry and Chemical Biology, 1280 Main Street West, Hamilton Ontario, Canada L8S 4K1 [3].
出版信息
Nat Commun. 2014 Jun 27;5:4279. doi: 10.1038/ncomms5279.
Catenanes are intriguing molecular assemblies for engineering unique molecular devices. The resident rings of a catenane are expected to execute unhindered rotation around each other, and to do so, they must have weak physical interactions with each other. Due to sequence programmability, DNA has become a popular material for nanoscale object engineering. However, current DNA catenanes, particularly in the single-stranded (ss) form, are synthesized through the formation of a linking duplex, which makes them less ideal as mobile elements for molecular machines. Herein we adopt a random library approach to engineer ssDNA [2] catenanes (two interlocked DNA rings) without a linking duplex. Results from DNA hybridization, double-stranded catenane synthesis and rolling circle amplification experiments signify that representative catenanes have weak physical interactions and are capable of operating as independent units. Our findings lay the foundation for exploring free-functioning interlocked DNA rings for the design of elaborate nanoscale machines based on DNA.
轮烷是一类有趣的分子组装体，可用于构建独特的分子器件。轮烷的内环被预期可以彼此不受阻碍地旋转，而为了实现这一点，它们彼此之间必须具有较弱的物理相互作用。由于序列可编程性，DNA 已成为用于纳米级物体工程的一种流行材料。然而，目前的 DNA 轮烷，特别是单链（ss）形式，是通过形成连接双链体来合成的，这使得它们作为分子机器的可移动元件不太理想。在此，我们采用随机文库的方法来构建没有连接双链体的 ssDNA [2] 轮烷（两个相互交织的 DNA 环）。DNA 杂交、双链体轮烷合成和滚环扩增实验的结果表明，代表性的轮烷具有较弱的物理相互作用，并且可以作为独立的单元运作。我们的发现为探索自由功能化的互锁 DNA 环奠定了基础，可用于设计基于 DNA 的精巧纳米级机器。
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