Natural Ingredient Development, Blue California, 30111 Tomas, Rancho Santa Margarita, CA 92688, USA.
Biomolecules. 2013 Sep 30;3(4):733-40. doi: 10.3390/biom3040733.
From the commercial extract of the leaves of Stevia rebaudiana Bertoni, a minor steviol glycoside, 13-[(2-O-β-D-glucopyranosyl-3-O-β-D-glucopyranosyl-β-D-glucopyranosyl)oxy] ent-kaur-16-en-19-oic acid-[(2-O-(3-O-β-D-glucopyranosyl-α-L-rhamnopyranosyl)-3-O-β-D-glucopyranosyl-β-D-glucopyranosyl) ester] (1); also known as rebaudioside O having seven sugar units has been isolated. Its structural characterization has been achieved by the extensive 1D (1H and 13C), and 2D NMR (COSY, HMQC, HMBC) as well as mass spectral data. Further, hydrolysis studies were performed on rebaudioside O using acid and enzymatic methods to identify aglycone and sugar residues in its structure as well as their configurations.
从甜菊叶的商业提取物中，分离得到一种微量甜菊糖苷，名为 13-[(2-O-β-D-吡喃葡萄糖基-3-O-β-D-吡喃葡萄糖基-β-D-吡喃葡萄糖基)氧基]贝壳杉-16-烯-19-酸-[(2-O-(3-O-β-D-吡喃葡萄糖基-α-L-鼠李吡喃糖基)-3-O-β-D-吡喃葡萄糖基-β-D-吡喃葡萄糖基)酯]（1）；它也被称为含有七个糖单元的莱鲍迪甙 O。通过广泛的 1D（1H 和 13C）和 2D NMR（COSY、HMQC、HMBC）以及质谱数据，实现了其结构表征。此外，还通过酸法和酶法对莱鲍迪甙 O 进行水解研究，以确定其结构中的糖苷配基和糖残基及其构型。
