He Canxia, Li Baolong, Song Wei, Ding Zhongqing, Wang Shuran, Shan Yujuan
School of Food Science and Engineering, Harbin Institute of Technology , No. 73 Huanghe Road, Harbin 150090, China.
J Agric Food Chem. 2014 Jul 30;62(30):7477-85. doi: 10.1021/jf501944u. Epub 2014 Jul 15.
In the present study, we investigated the potential efficacy of cruciferous vegetable-derived sulforaphane (SFN) in improving homocysteine (HCY)-stressed cells. After human hepatocyte line HHL-5 was preincubated with SFN and subsequently with 10 mmol/L HCY, SFN improved the pathologic changes which are caused by HCY, including cell morphological abnormality, endoplasmic reticulum (ER) swelling, excessive generation of reactive oxygen species (ROS), the increased malondialdehyde (MDA) levels, as well as the increased activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST). Phase II enzymes, thioredoxin reductase-1 (TrxR-1) and
NAD(P)H: quinone oxidoreductase 1 (NQO1), were involved in the protective effect of SFN against injury by HCY. The ER stress-specific proteins, such as glucose-regulated protein-78 (GRP78) and protein kinase RNA (PKR)-like ER kinase (PERK), were strikingly abolished by SFN. Furthermore, Nrf-2 translocation was enhanced by SFN, which lead to the induction of TrxR-1and NQO1.
在本研究中，我们调查了十字花科蔬菜来源的萝卜硫素（SFN）在改善同型半胱氨酸（HCY）应激细胞方面的潜在功效。在人肝细胞系HHL-5先用SFN预孵育，随后用10 mmol/L HCY处理后，SFN改善了由HCY引起的病理变化，包括细胞形态异常、内质网（ER）肿胀、活性氧（ROS）过度生成、丙二醛（MDA）水平升高以及丙氨酸转氨酶（ALT）和天冬氨酸转氨酶（AST）活性增加。II期酶硫氧还蛋白还原酶-1（TrxR-1）和NAD（P）H：醌氧化还原酶1（NQO1）参与了SFN对HCY损伤的保护作用。SFN显著消除了内质网应激特异性蛋白，如葡萄糖调节蛋白78（GRP78）和蛋白激酶RNA（PKR）样内质网激酶（PERK）。此外，SFN增强了Nrf-2易位，这导致了TrxR-1和NQO1的诱导。
