University of Minnesota Veterinary Diagnostic Laboratory, Saint Paul, MN, United States.
Viral Disease and Epidemiology Research Division, National Institute of Animal Health, National Agriculture and Food Research Organization, Ibaraki, Japan.
Vet Microbiol. 2014 Aug 27;172(3-4):359-66. doi: 10.1016/j.vetmic.2014.05.015. Epub 2014 Jun 2.
Rotavirus B (RVB) has been identified as a causative agent of diarrhea in rats, humans, cattle, lambs, and swine. Recently, 20 RVB VP7 genotypes were determined based on an 80% nucleotide percent cut-off value. In this study, we sequenced the RVB VP6 gene segment from 80 RVB positive swine samples from the United States and Japan. Phylogenetic analyses, using the 30 available RVB VP6 sequences from GenBank and our 80 novel RVB VP6 sequences, revealed a large genetic diversity of RVB strains, mainly in pigs. For classification purposes, pairwise identity frequency analyses suggested an 81% nucleotide percent cut-off value, resulting in 13 RVB VP6 (I) genotypes. In addition, an intragenic recombinant RVB VP6 segment was identified from Japan. Furthermore, the data indicates frequent reassortment events occurred between the porcine RVB VP7 and VP6 gene segments.
轮状病毒 B（RVB）已被确定为导致大鼠、人类、牛、羔羊和猪腹泻的病原体。最近，根据 80%核苷酸百分比截断值，确定了 20 种 RVB VP7 基因型。在这项研究中，我们对来自美国和日本的 80 个 RVB 阳性猪样本的 RVB VP6 基因片段进行了测序。使用从 GenBank 获得的 30 个可用的 RVB VP6 序列和我们的 80 个新的 RVB VP6 序列进行系统发育分析，结果表明 RVB 株具有很大的遗传多样性，主要在猪中。为了分类目的，对核苷酸相似度频率的分析表明，81%的核苷酸百分比截断值导致了 13 种 RVB VP6（I）基因型。此外，在日本发现了一个 RVB VP6 基因内重组片段。此外，数据表明，猪 RVB VP7 和 VP6 基因片段之间经常发生重配事件。
