Alekseev Konstantin P, Penin Aleksey A, Mukhin Alexey N, Khametova Kizkhalum M, Grebennikova Tatyana V, Yuzhakov Anton G, Moskvina Anna S, Musienko Maria I, Raev Sergey A, Mishin Alexandr M, Kotelnikov Alexandr P, Verkhovsky Oleg A, Aliper Taras I, Nepoklonov Eugeny A, Herrera-Ibata Diana M, Shepherd Frances K, Marthaler Douglas G
N. F. Gamaleya National Research Center for Epidemiology and Microbiology, Gamaleya Str. 18, Moscow 123098, Russia.
Federal State Budget Scientific Institution "Federal Scientific Centre VIEV", Moscow 109428, Russia.
Pathogens. 2018 Apr 20;7(2):46. doi: 10.3390/pathogens7020046.
An outbreak of enteric disease of unknown etiology with 60% morbidity and 8% mortality in weaning piglets occurred in November 2015 on a farm in Buryat Republic, Russia. Metagenomic sequencing revealed the presence of rotavirus B in feces from diseased piglets while no other pathogens were identified. Clinical disease was reproduced in experimentally infected piglets, yielding the 11 RVB gene segments for strain Buryat15, with an RVB genotype constellation of G12-P[4]-I13-R4-C4-M4-A8-N10-T4-E4-H7. This genotype constellation has also been identified in the United States. While the Buryat15 VP7 protein lacked unique amino acid differences in the predicted neutralizing epitopes compared to the previously published swine RVB G12 strains, this report of RVB in Russian swine increases our epidemiological knowledge on the global prevalence and genetic diversity of RVB.
2015年11月，俄罗斯布里亚特共和国的一个农场发生了一起病因不明的肠道疾病暴发，断奶仔猪发病率为60%，死亡率为8%。宏基因组测序显示，患病仔猪粪便中存在轮状病毒B，未发现其他病原体。在实验感染的仔猪中再现了临床疾病，获得了布里亚特15株的11个RVB基因片段，RVB基因型组合为G12-P[4]-I13-R4-C4-M4-A8-N10-T4-E4-H7。这种基因型组合在美国也有发现。虽然与之前发表的猪RVB G12株相比，布里亚特15株VP7蛋白在预测的中和表位中没有独特的氨基酸差异，但这份关于俄罗斯猪中RVB的报告增加了我们对RVB全球流行率和遗传多样性的流行病学认识。
