Mycology Laboratory, National Veterinary Research Institute, Vom, Nigeria.
Department of Veterinary Microbiology, Faculty of Veterinary Medicine, Ahmadu bello University, Zaria, Nigeria.
Vet Microbiol. 2014 Aug 27;172(3-4):475-8. doi: 10.1016/j.vetmic.2014.05.014. Epub 2014 May 13.
Sporothrix schenkii and Dermatophilus congolensis were isolated from a bull with severe generalized skin lesions. The lesions were thick, crusty and scabby on the dorsal part while few scabby and several nodular lesions were seen on the lower limbs especially the thighs. Scab samples and exudates from the nodules were aseptically collected and processed for bacteriology and mycology. Gram stained smears revealed Gram-positive, filamentous organism that had longitudinal and transverse septa suggestive of D. congolensis. Colonies on 5% defibrinated sheep blood agar were small, rough, grayish-white, β-hemolytic and adherent to the medium. It was catalase positive, urease positive and fermented glucose and maltose but not sucrose, lactose, mannitol, sorbitol and xylose. Colonies on Sabouraud's dextrose agar were small, round, white and opaque, delicate and smooth. It liquefied gelatin and fermented glucose and sucrose but not galactose, menite, and glycerin. The isolate was Gram-positive, cigar-shaped and yeast-like suggestive of S. schenkii. Dermatophilosis is common in domesticated ruminants while sporotrichosis is very rare in cattle. This may be the first report of bovine sporotrichosis from Africa.
从一头患有严重全身性皮肤病变的公牛身上分离出了申克孢子丝菌和刚果嗜皮菌。病变的背部皮肤厚、结痂且有鳞屑，而下肢尤其是大腿上则有少量结痂和几个结节性病变。无菌采集结痂样本和结节渗出物，进行细菌学和真菌学检查。革兰氏染色涂片显示革兰氏阳性、丝状生物体，具有纵向和横向隔膜，提示为刚果嗜皮菌。在 5%去纤维绵羊血琼脂上的菌落小、粗糙、灰白色、β-溶血且与培养基黏附。它呈过氧化氢酶阳性、脲酶阳性，能发酵葡萄糖和麦芽糖，但不能发酵蔗糖、乳糖、甘露醇、山梨醇和木糖。在沙氏葡萄糖琼脂上的菌落小、圆形、白色、不透明、细腻光滑。它能液化明胶并发酵葡萄糖和蔗糖，但不能发酵半乳糖、麦芽糖和甘油。该分离株呈革兰氏阳性、雪茄形和酵母样，提示为申克孢子丝菌。嗜皮菌病在家养反刍动物中很常见，而牛的孢子丝菌病则非常罕见。这可能是非洲首例牛的孢子丝菌病报告。
