天然冰片与姜黄素对A375人黑色素瘤细胞的协同诱导凋亡作用。
Synergistic apoptosis-inducing effects on A375 human melanoma cells of natural borneol and curcumin.
作者信息
Chen Jianping, Li Lin, Su Jianyu, Li Bing, Chen Tianfeng, Wong Yum-Shing
机构信息
College of Light Industry and Food Sciences, South China University of Technology, Guangzhou, China.
College of Light Industry and Food Sciences, South China University of Technology, Guangzhou, China; Guangdong Hua Qing Yuan Biological Technology Co., Ltd., Meizhou, China.
出版信息
PLoS One. 2014 Jun 27;9(6):e101277. doi: 10.1371/journal.pone.0101277. eCollection 2014.
This study was to investigate the synergistic effect of NB/Cur on growth and apoptosis in A375 human melanoma cell line by MTT assay, flow cytometry and Western blotting. Our results demonstrated that NB effectively synergized with Cur to enhance its antiproliferative activity on A375 human melanoma cells by induction of apoptosis, as evidenced by an increase in sub-G1 cell population, DNA fragmentation, PARP cleavage and caspase activation. Further mechanistic studies by Western blotting showed that after treatment of the cells with NB/Cur, up-regulation of the expression level of phosphorylated JNK and down-regulation of the expression level of phosphorylated ERK and Akt contributed to A375 cells apoptosis. Moreover, NB also potentiated Cur to trigger intracellular ROS overproduction and the DNA damage with up-regulation of the expression level of phosphorylated ATM, phosphorylated Brca1 and phosphorylated p53. The results indicate the combinational application potential of NB and Cur in treatments of cancers.
本研究旨在通过MTT法、流式细胞术和蛋白质免疫印迹法研究NB/Cur对A375人黑色素瘤细胞系生长和凋亡的协同作用。我们的结果表明，NB与Cur有效协同，通过诱导凋亡增强其对A375人黑色素瘤细胞的抗增殖活性，亚G1期细胞群增加、DNA片段化、PARP裂解和半胱天冬酶激活证明了这一点。蛋白质免疫印迹法进一步的机制研究表明，用NB/Cur处理细胞后，磷酸化JNK表达水平上调，磷酸化ERK和Akt表达水平下调导致A375细胞凋亡。此外，NB还增强了Cur引发细胞内ROS过量产生和DNA损伤的作用，同时磷酸化ATM、磷酸化Brca1和磷酸化p53的表达水平上调。结果表明NB和Cur在癌症治疗中具有联合应用潜力。
Zotero 插件