Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Provincial Engineering Technology Research Center of Seafood, Guangdong Province Engineering Laboratory for Marine Biological Products, Key Laboratory of Advanced Processing of Aquatic Product of Guangdong Higher Education Institution, Guangdong Provincial Science and Technology Innovation Center for Subtropical Fruit and Vegetable Processing, College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China.
Collaborative Innovation Center of Seafood Deep Processing, Dalian Polytechnic University, Dalian 116034, China.
Molecules. 2023 Mar 13;28(6):2610. doi: 10.3390/molecules28062610.
Fucoidan has many biological functions, including anti-tumor activity. Additionally, it has been suggested that low-molecular-weight fucoidans have greater bioactivities. This study aimed to examine the degradation, purification, physicochemical characterization and in vitro antitumor activity of fucoidan from (Turner) C. Agardh. Fucoidan was isolated using DEAE-cellulose-52 (F1, F2), Vc-HO degration, and Sepharose CL-6B gel (DF1, DF2) from crude Sargassum fucoidans. Physicochemical characteristics of four isolated fucoidans were examined using chemical and monosaccharide composition, average molecular weight (Mw), and FTIR. Furthermore, the anti-proliferative effects of purified fucoidans on human hepatocellular carcinoma cells (HepG2), human Burkitt Lymphoma cells (MCF-7), human uterine carcinoma cells (Hela) and human lung cancer cells (A549) were analyzed by MTT method. The apoptosis of HepG2 cells was detected by flow cytometry. Our data suggest that the contents of polysaccharide, L-fucose and sulfate of DF2 were the highest, which were 73.93%, 23.02% and 29.88%, respectively. DF1 has the smallest molecular weight (14,893 Da) followed by DF2 (21,292 Da). The four fractions are mainly composed of fucose, mannose and rhamnose, and the infrared spectra are similar, all of which contain polysaccharide and sulfate characteristic absorption peaks. The results of MTT assay showed that the four fractions had inhibitory effects on HepG2 and A549 in the range of 0.5-8 mg/mL, and the four fractions had strong cytotoxic effects on HepG2 cells. DF2 had the best inhibitory effect on HepG2 (IC = 2.2 mg/mL). In general, the antitumor activity of Sargassum fucoidans is related to the content of L-fucose, sulfate and molecular weight, and Sargassum fucoidan has the best inhibitory effect on HepG2 hepatocellular carcinoma cells. Furthermore, when compared to MCF-7, Hela, and A549 cells, Sargassum fucoidans had the best capacity to reduce the viability of human hepatocellular carcinoma cells (HepG2) and to induce cell apoptosis, proving itself to have a good potential in anti-liver cancer therapy.
岩藻聚糖具有多种生物功能，包括抗肿瘤活性。此外，低分子量岩藻聚糖具有更大的生物活性。本研究旨在研究从（特纳）C. 海藻中提取的岩藻聚糖的降解、纯化、理化特性及体外抗肿瘤活性。使用 DEAE-纤维素-52（F1、F2）、Vc-HO 降解和 Sepharose CL-6B 凝胶（DF1、DF2）从粗岩藻聚糖中分离岩藻聚糖。通过化学和单糖组成、平均分子量（Mw）和傅里叶变换红外光谱（FTIR）对四种分离岩藻聚糖的理化特性进行了研究。此外，采用 MTT 法分析了纯化岩藻聚糖对人肝癌细胞（HepG2）、人 Burkitt 淋巴瘤细胞（MCF-7）、人子宫颈癌细胞（Hela）和人肺癌细胞（A549）的增殖抑制作用。通过流式细胞术检测 HepG2 细胞的凋亡。我们的数据表明，DF2 的多糖、L-岩藻糖和硫酸根含量最高，分别为 73.93%、23.02%和 29.88%。DF1 的分子量最小（14893 Da），其次是 DF2（21292 Da）。四个馏分主要由岩藻糖、甘露糖和鼠李糖组成，红外光谱相似，均含有多糖和硫酸根特征吸收峰。MTT 法结果表明，四个馏分在 0.5-8 mg/mL 范围内对 HepG2 和 A549 均有抑制作用，四个馏分对 HepG2 细胞均有较强的细胞毒性作用。DF2 对 HepG2 的抑制作用最好（IC=2.2 mg/mL）。总体而言，岩藻聚糖的抗肿瘤活性与 L-岩藻糖、硫酸根和分子量的含量有关，而岩藻聚糖对 HepG2 肝癌细胞的抑制作用最好。此外，与 MCF-7、Hela 和 A549 细胞相比，岩藻聚糖降低人肝癌细胞（HepG2）活力和诱导细胞凋亡的能力最强，证明其在肝癌治疗中有很好的应用潜力。
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