Mihatovic I, Payer M, Bertrams M, Vasiliu D, Schwarz F, Becker J, Stratul S I
Department of Oral Surgery, Heinrich Heine University, Westdeutsche Kieferklinik, Moorenstr. 5, D-40225 Düsseldorf, Germany.
Department of Oral Surgery and Radiology, School of Dentistry, Medical University of Graz, Austria.
J Craniomaxillofac Surg. 2014 Oct;42(7):1171-7. doi: 10.1016/j.jcms.2014.02.004. Epub 2014 Mar 10.
The present study was conducted to evaluate the bone tissue response after the application of an oily calcium hydroxide suspension (OCHS) into defects created in the tibial bone of minipigs.
Standardized defects (2 ccm) were created into the tibia of 4 Goettinger minipigs. Defects in the test group (n = 4) were filled with OCHS (Osteora, DFS-Diamon, Riedenburg, Germany). Defects in the control group (n = 4) were filled with venous blood. Animals were sacrificed after healing periods of 4 and 8 weeks. Tibias were dissected, soft tissues removed and processed for histological analysis. Digital images (×200) were evaluated using the software CellD (Soft Imaging System, Münster, Germany). The following histomorphometrical landmarks were identified: defect size, mineralized tissue, non-mineralized tissue and residual OCHS.
Healing was uneventful in all four animals. In the test group, new bone formation was observed in the vicinity of the defect margins whereas the centre of the defect was dominated by non-mineralized tissue. Mean percentages of mineralized tissue after 4 weeks were 23.01% in the test group vs. 43.45% in the control group. The mean value for residual OCHS was 7,11% at 4 weeks. After 8 weeks mean percentages of mineralized tissue were 28.15% in the test group vs. 44.39% in the control group as well as 7.05% for residual OCHS.
Within the limits of the present pilot study it can be concluded that OCHS did not have a beneficial effect on new bone formation. To prove an osteoinductive potential of OCHS further studies based on a higher number of samples are needed.
本研究旨在评估将油性氢氧化钙混悬液（OCHS）应用于小型猪胫骨缺损后骨组织的反应。
在4只哥廷根小型猪的胫骨上制造标准化缺损（2立方厘米）。试验组（n = 4）的缺损用OCHS（Osteora，DFS-Diamon，德国里登堡）填充。对照组（n = 4）的缺损用静脉血填充。在4周和8周的愈合期后处死动物。解剖胫骨，去除软组织并进行组织学分析。使用CellD软件（德国明斯特的Soft Imaging System）评估数字图像（×200）。确定了以下组织形态计量学指标：缺损大小、矿化组织、非矿化组织和残留的OCHS。
所有四只动物的愈合过程均顺利。在试验组中，在缺损边缘附近观察到新骨形成，而缺损中心以非矿化组织为主。4周后，试验组矿化组织的平均百分比为23.01%，而对照组为43.45%。4周时残留OCHS的平均值为7.11%。8周后，试验组矿化组织的平均百分比为28.15%，对照组为44.39%，残留OCHS为7.05%。
在本初步研究的范围内，可以得出结论，OCHS对新骨形成没有有益作用。要证明OCHS的骨诱导潜力，需要基于更多样本进行进一步研究。
