Stavropoulos Andreas, Geenen Claudia, Nyengaard Jens R, Karring Thorkild, Sculean Anton
Department of Periodontology and Oral Gerontology, Royal Dental College, University of Aarhus, Aarhus, Denmark.
Clin Oral Implants Res. 2007 Dec;18(6):761-7. doi: 10.1111/j.1600-0501.2007.01403.x. Epub 2007 Sep 14.
To evaluate whether an oily calcium hydroxide suspension (OCHS) promotes bone healing when used as an adjunct to guided bone regeneration (GBR).
Rigid, hemispherical, teflon capsules were placed with their open part facing the lateral surface of the ramus on both sides of the mandible in 10 adult Wistar rats. In each animal, one capsule was filled out with an OCHS (test) before placement, while the capsule on the other side was left empty (control). After 4 months of healing, the animals were sacrificed and histological sections containing the capsules and the neighboring soft and hard tissues were prepared. On three to four sections taken by uniformly random sampling from each specimen, the relative volumes of (1) the newly formed bone (mineralized bone and marrow), (2) the soft connective tissue, (3) the residual OCHS, and (4) the acellular (empty) space inside the capsule were estimated by a point-counting technique, and expressed as percentage of the space originally created by the capsule.
There was no new bone formation inside the capsules in all but one test specimen, where only a minimal amount of newly formed bone could be observed in continuation with the lateral surface of the ramus. OCHS had a homogenous appearance and occupied the major portion (79.4%) of the space created by the capsule. No signs of active resorption of the material could be observed. On the contrary, 31.5% of the space provided by the capsule was filled out with newly formed bone in the control group. The new bone had a trabecular appearance with large marrow spaces filled with hematopoietic and fatty marrow. The rest of the capsule space in the controls appeared empty.
OCHS may hamper bone healing when used as an adjunct to GBR.
评估油性氢氧化钙悬液（OCHS）作为引导骨再生（GBR）辅助材料时是否能促进骨愈合。
将硬质半球形聚四氟乙烯胶囊开口部分朝下放置在10只成年Wistar大鼠下颌骨两侧升支的外侧表面。在每只动物中，一个胶囊在放置前填充OCHS（试验组），而另一侧的胶囊保持为空（对照组）。愈合4个月后，处死动物，制备包含胶囊及相邻软硬组织的组织学切片。从每个标本中均匀随机抽取三到四张切片，采用点计数技术估计（1）新形成骨（矿化骨和骨髓）、（2）软结缔组织、（3）残余OCHS和（4）胶囊内无细胞（空）空间的相对体积，并表示为胶囊最初所占据空间的百分比。
除一个试验标本外，所有胶囊内均未形成新骨，在该试验标本中，仅在与升支外侧表面连续处观察到少量新形成骨。OCHS外观均匀，占据胶囊所形成空间的主要部分（79.4%）。未观察到该材料有活跃吸收的迹象。相反，对照组中胶囊所提供空间的31.5%被新形成骨填充。新骨呈小梁状外观，有大的骨髓腔，充满造血和脂肪骨髓。对照组中胶囊其余空间看起来为空。
OCHS作为GBR辅助材料使用时可能会妨碍骨愈合。
