Yee J B, Hutson J C
Department of Cell Biology and Anatomy, Texas Tech University Health Sciences Center, Lubbock 79430.
Life Sci. 1989;44(17):1193-9. doi: 10.1016/0024-3205(89)90314-7.
Sertoli cells of the testis secrete lactate in response to follicle-stimulating hormone (FSH). It is thought that the developing germ cells use lactate as an energy substrate preferentially over glucose. However, the biochemical mechanism(s) involved in the regulation of lactate secretion in response to FSH are unknown. The purpose of this study was to determine if extracellular calcium was important for the actions of FSH during this response. It was found that the FSH-induced increase in lactate production by Sertoli cells was not dependent upon the presence of extracellular calcium. However, A23187 (a calcium ionophore) stimulated lactate secretion in the presence of extracellular calcium. When FSH and A23187 were tested together at maximal concentrations, more lactate was secreted than when either FSH or A23187 was tested alone. Neither verapamil, nifedipine nor diltiazem (calcium channel "blockers") were able to inhibit the ability of FSH to increase lactate secretion. These results indicate that FSH-induced secretion of lactate by cultured Sertoli cells is not dependent upon extracellular calcium.
睾丸的支持细胞会响应促卵泡激素（FSH）分泌乳酸。据认为，发育中的生殖细胞优先将乳酸用作能量底物，而非葡萄糖。然而，参与FSH响应调节乳酸分泌的生化机制尚不清楚。本研究的目的是确定细胞外钙在FSH此响应过程中的作用是否重要。结果发现，FSH诱导支持细胞乳酸生成增加并不依赖于细胞外钙的存在。然而，A23187（一种钙离子载体）在细胞外钙存在的情况下会刺激乳酸分泌。当以最大浓度同时测试FSH和A23187时，分泌的乳酸比单独测试FSH或A23187时更多。维拉帕米、硝苯地平或地尔硫䓬（钙通道“阻滞剂”）均无法抑制FSH增加乳酸分泌的能力。这些结果表明，培养的支持细胞中FSH诱导的乳酸分泌不依赖于细胞外钙。
