Mita M, Price J M, Hall P F
Endocrinology. 1982 May;110(5):1535-41. doi: 10.1210/endo-110-5-1535.
Sertoli cells from rats aged 16 days were cultured in defined medium for 2 days and then treated by addition of fresh medium containing various hormones (treated) or saline (control). The concentration of lactate in the medium was measured as a function of time. The production of lactate measured under these conditions was increased by addition of FSH to the medium. For NIH FSH (13 and 14), ED50 for stimulation of lactate production was approximately 0.05 micrograms/ml. Stimulation was also seen with LH and TSH (ED50, 0.8 micrograms/ml for both hormones). Reasons are given for believing that TSH may possess the inherent capacity to stimulate production of lactate in contrast to LH, which appears to act only by way of contaminating traces of FSH. Dibutyryl cAMP also stimulates lactate production by Sertoli cells. Other hormones tested, including androgens, were without effect. When production of lactate by Sertoli cells was compared with the maximal consumption by spermatids in vitro, it became apparent that Sertoli cells may provide the major source of metabolic substrate for spermatids in the form of lactate. Stimulation of lactate production by FSH was inhibited by puromycin, cycloheximide, and actinomycin D. Evidently this response requires synthesis of new protein and RNA. This effect of FSH may be important in the regulation of spermatogenesis.
将16日龄大鼠的支持细胞在限定培养基中培养2天，然后通过添加含有各种激素的新鲜培养基（处理组）或生理盐水（对照组）进行处理。测定培养基中乳酸浓度随时间的变化。在这些条件下，向培养基中添加促卵泡激素（FSH）可增加乳酸的产生。对于美国国立卫生研究院（NIH）的FSH（13和14），刺激乳酸产生的半数有效剂量（ED50）约为0.05微克/毫升。促黄体生成素（LH）和促甲状腺激素（TSH）也有刺激作用（两种激素的ED50均为0.8微克/毫升）。有理由认为，与似乎仅通过污染的微量FSH起作用的LH不同，TSH可能具有刺激乳酸产生的内在能力。二丁酰环磷腺苷（Dibutyryl cAMP）也可刺激支持细胞产生乳酸。所测试的其他激素，包括雄激素，均无作用。当将支持细胞产生乳酸的量与体外精子细胞的最大消耗量进行比较时，很明显支持细胞可能以乳酸的形式为精子细胞提供主要的代谢底物来源。嘌呤霉素、放线菌酮和放线菌素D可抑制FSH对乳酸产生的刺激作用。显然，这种反应需要合成新的蛋白质和RNA。FSH的这种作用在精子发生的调节中可能很重要。
