Brinkmalm Ann, Brinkmalm Gunnar, Honer William G, Moreno Julie A, Jakobsson Joel, Mallucci Giovanna R, Zetterberg Henrik, Blennow Kaj, Öhrfelt Annika
From the ‡Department of Psychiatry and Neurochemistry, Institute of Neuroscience and Physiology, Sahlgrenska Academy at the University of Gothenburg, S43180 Mölndal, Sweden;
¶Department of Psychiatry, University of British Columbia, Vancouver V6H3Z6, British Columbia, Canada;
Mol Cell Proteomics. 2014 Oct;13(10):2584-92. doi: 10.1074/mcp.M114.040113. Epub 2014 Jun 27.
We report a novel strategy for studying synaptic pathology by concurrently measuring levels of four SNARE complex proteins from individual brain tissue samples. This method combines affinity purification and mass spectrometry and can be applied directly for studies of SNARE complex proteins in multiple species or modified to target other key elements in neuronal function. We use the technique to demonstrate altered levels of presynaptic proteins in Alzheimer disease patients and prion-infected mice.
我们报告了一种通过同时测量来自个体脑组织样本的四种SNARE复合体蛋白水平来研究突触病理学的新策略。该方法结合了亲和纯化和质谱技术,可直接应用于多种物种的SNARE复合体蛋白研究,或进行修改以靶向神经元功能中的其他关键元件。我们使用该技术证明了阿尔茨海默病患者和朊病毒感染小鼠中突触前蛋白水平的改变。