Program of Petrochemical and Polymer Science, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand; Program of Chemistry, Faculty of Science and Technology, Nakhon Si Thammarat Rajabhat University, Nakhon Si Thammarat 80280, Thailand.
Organic Synthesis Research Unit, Department of Chemistry, Faculty of Science and Nanotec-CU Center of Excellent on Food and Agriculture, Chulalongkorn University, Bangkok 10330, Thailand.
Biosens Bioelectron. 2014 Dec 15;62:8-12. doi: 10.1016/j.bios.2014.05.069. Epub 2014 Jun 12.
Widespread use of dichlorvos in agriculture has posed serious concern for food and water contamination. A new colorimetric method for the detection of dichlorvos based on polydiacethylene and acetylcholinesterase inhibition is developed. The blue-to-red color transition of poly(10,12-pentacosadynoic acid) vesicles can be induced by myristoylcholine which is enzymatically hydrolyzed by acetylcholinesterase to myristic acid and choline to prevent the color transition. In the presence of dichlorvos, the hydrolytic activity of the enzyme is inhibited that the blue-to-red color transition is restored with a linear correlation to the dichlorvos concentration. Using UV-vis absorption spectrometer, the limit of dichlorvos detection is 6.7 ppb. A naked eye detection of 50 ppb dichlorvos is achievable by using dimiristoylphosphatidylcholine to the diacetylene mixed lipid vesicles.
二氯松在农业中的广泛使用对食品和水的污染造成了严重的担忧。基于聚二乙酰亚胺和乙酰胆碱酯酶抑制作用，开发了一种检测二氯松的新比色法。酰基肉毒碱可以诱导 10,12-二十五碳二烯酸囊泡的蓝-红颜色转变，该酰基肉毒碱可被乙酰胆碱酯酶酶解为肉豆蔻酸和胆碱，从而阻止颜色转变。在二氯松存在的情况下，酶的水解活性受到抑制，蓝色到红色的颜色转变恢复，与二氯松浓度呈线性相关。使用紫外可见吸收光谱仪，二氯松的检测限为 6.7 ppb。使用二肉豆蔻酰磷脂酰胆碱到二乙酰基混合脂质囊泡，可以实现对 50 ppb 二氯松的肉眼检测。
