Peng Shu, Pan Yu-Chen, Wang Yaling, Xu Zhe, Chen Chao, Ding Dan, Wang Yongjian, Guo Dong-Sheng
College of ChemistryState Key Laboratory of Elemento-Organic Chemistry Key Laboratory of Functional Polymer Materials Ministry of Education Nankai University Tianjin 300071 China.
Key Laboratory of Bioactive Materials Ministry of Education College of Life Sciences Nankai University Tianjin 300071 China.
Adv Sci (Weinh). 2017 Aug 10;4(11):1700310. doi: 10.1002/advs.201700310. eCollection 2017 Nov.
The introduction of controlled self-assembly into living organisms opens up desired biomedical applications in wide areas including bioimaging/assays, drug delivery, and tissue engineering. Besides the enzyme-activated examples reported before, controlled self-assembly under integrated stimuli, especially in the form of sequential input, is unprecedented and ultimately challenging. This study reports a programmable self-assembling strategy in living cells under sequentially integrated control of both endogenous and exogenous stimuli. Fluorescent polymerized vesicles are constructed by using cholinesterase conversion followed by photopolymerization and thermochromism. Furthermore, as a proof-of-principle application, the cell apoptosis involved in the overexpression of cholinesterase in virtue of the generated fluorescence is monitored, showing potential in screening apoptosis-inducing drugs. The approach exhibits multiple advantages for bioimaging in living cells, including specificity to cholinesterase, red emission, wash free, high signal-to-noise ratio.
将可控自组装引入活生物体，为生物成像/分析、药物递送和组织工程等广泛领域带来了理想的生物医学应用。除了之前报道的酶激活实例外，在综合刺激下，尤其是以顺序输入的形式进行的可控自组装，是前所未有的，也是极具挑战性的。本研究报道了一种在活细胞中，在内源性和外源性刺激的顺序综合控制下的可编程自组装策略。通过胆碱酯酶转化，然后进行光聚合和热致变色，构建了荧光聚合囊泡。此外，作为原理验证应用，借助产生的荧光监测了胆碱酯酶过表达所涉及的细胞凋亡，显示出在筛选凋亡诱导药物方面的潜力。该方法在活细胞生物成像方面具有多种优势，包括对胆碱酯酶的特异性、红色发射、无需洗涤、高信噪比。
