Department of Biology, Systemic Physiological and Ecotoxicological Research (SPHERE), University of Antwerp, Groenenborgerlaan 171, 2020 Antwerp, Belgium.
Department of Biology, Evolutionary Ecology Group, University of Antwerp, Groenenborgerlaan 171, 2020 Antwerp, Belgium; Research Institute of Nature and Forest (INBO), Kliniekstraat 25, 1070 Brussel, Belgium.
Aquat Toxicol. 2014 Sep;154:270-7. doi: 10.1016/j.aquatox.2014.05.028. Epub 2014 Jun 4.
The present study measured various pesticides and trace metals, together with sublethal effect biomarkers (lipid, protein and glycogen levels, acetylcholinesterase (AChE) and glutathione-S-transferase (GST) activities) in damselfly larvae (Ischnura elegans) at sixteen sampling sites in Flanders (Belgium). Four pesticides (chloridazon, dichlorvos, terbutylazine, metolachlor), some of them hardly measurable in surface water, and all trace metals were above the limit of quantification in damselfly tissue. A principal component analysis (PCA) on the accumulated pollutant concentrations returned five pollutant axes explaining 85.8% of the total variation. Based on these PCA-axes a hierarchical cluster analysis revealed that the 16 sampled ponds could be classified in 7 groups. Increasing dichlorvos levels in the animals resulted in a lower body mass. Body mass was negatively correlated with GST and AChE activities, lipid and glycogen levels. The present findings provide evidence of toxicity-induced sublethal stress of dichlorvos accumulation in natural populations of I. elegans.
本研究在比利时弗兰德斯的 16 个采样点测量了各种杀虫剂和痕量金属，以及亚致死效应生物标志物（脂质、蛋白质和糖原水平、乙酰胆碱酯酶（AChE）和谷胱甘肽-S-转移酶（GST）活性）在豆娘幼虫（Ischnura elegans）中。四种杀虫剂（氯唑嗪、敌百虫、特丁津、甲草胺），其中一些在地表水几乎无法测量，所有痕量金属在豆娘组织中的含量均超过定量限。基于累积污染物浓度的主成分分析（PCA）得到了五个解释总变异 85.8%的污染物轴。基于这些 PCA 轴，层次聚类分析将 16 个采样池塘分为 7 组。动物体内敌百虫水平的升高导致体重降低。体重与 GST 和 AChE 活性、脂质和糖原水平呈负相关。本研究结果为自然种群中 I. elegans 敌百虫积累引起的毒性诱导亚致死应激提供了证据。
