定量荧光显微镜技术的相关概念。

Concepts in quantitative fluorescence microscopy.

作者信息

Waters Jennifer C, Wittmann Torsten

机构信息

Department of Cell Biology, Harvard Medical School, Boston, Massachusetts, USA.

Department of Cell and Tissue Biology, University of California, San Francisco, USA.

出版信息

Methods Cell Biol. 2014;123:1-18. doi: 10.1016/B978-0-12-420138-5.00001-X.

DOI:10.1016/B978-0-12-420138-5.00001-X

PMID:24974019

Abstract

In recent years, there has been an enormous increase in the publication of spatial and temporal measurements made on fluorescence microscopy digital images. Quantitative fluorescence microscopy is a powerful and important tool in biological research but is also an error-prone technique that requires careful attention to detail. In this chapter, we focus on general concepts that are critical to performing accurate and precise quantitative fluorescence microscopy measurements.

摘要

近年来，对荧光显微镜数字图像进行的空间和时间测量的出版物数量大幅增加。定量荧光显微镜是生物学研究中一种强大且重要的工具，但也是一种容易出错的技术，需要对细节予以仔细关注。在本章中，我们将重点关注对于进行准确和精确的定量荧光显微镜测量至关重要的一般概念。