Goodwin Paul C
GE Healthcare, Issaquah, Washington, USA; Department of Comparative Medicine, University of Washington, Seattle, Washington, USA.
Methods Cell Biol. 2014;123:177-92. doi: 10.1016/B978-0-12-420138-5.00010-0.
The light microscope is an essential tool for the study of cells, organelles, biomolecules, and subcellular dynamics. A paradox exists in microscopy whereby the higher the needed lateral resolution, the more the image is degraded by out-of-focus information. This creates a significant need to generate axial contrast whenever high lateral resolution is required. One strategy for generating contrast is to measure or model the optical properties of the microscope and to use that model to algorithmically reverse some of the consequences of high-resolution imaging. Deconvolution microscopy implements model-based methods to enable the full diffraction-limited resolution of the microscope to be exploited even in complex and living specimens.
光学显微镜是研究细胞、细胞器、生物分子和亚细胞动力学的重要工具。显微镜领域存在一个悖论，即所需的横向分辨率越高，离焦信息对图像的降解就越严重。因此，每当需要高横向分辨率时，就迫切需要产生轴向对比度。产生对比度的一种策略是测量或建模显微镜的光学特性，并使用该模型通过算法来逆转高分辨率成像的一些影响。反卷积显微镜采用基于模型的方法，即使在复杂的活体样本中也能充分利用显微镜的全衍射极限分辨率。
