Zhang Xiao-Jun, Su Yao-Rong, Liu Dan, Xu De-Bin, Zeng Mu-Sheng, Chen Wen-Kuan
Department of Head and Neck Surgery, Sun Yat-sen University Cancer Center, Guangzhou, P. R. China; State Key Laboratory of Oncology in South China, Guangzhou, P. R. China; Collaborative Innovation Center for Cancer Medicine, Guangzhou, P. R. China.
Department of General Surgery, Jiangmen Central Hospital, Jiangmen, P. R. China.
J Surg Res. 2014 Dec;192(2):487-93. doi: 10.1016/j.jss.2014.05.066. Epub 2014 May 27.
Thymosin beta 10 (TMSB10) has recently been recognized as being an important player in the metastatic cascade including tumor angiogenesis, invasion, and metastasis. However, a role for this protein in papillary thyroid carcinoma (PTC) has not yet been established.
Real-time polymerase chain reaction was used to examine the expression of TMSB10 messenger RNA in 36 cases of thyroid tissue samples: normal thyroid, PTC without lymph node metastases (LNM) and PTC with LNM (n = 12 cases in each subgroup). For immunohistochemistry, 130 patients with PTC were selected during the period of 2004-2005, 91 with and 39 without LNM. Statistical analysis was applied to evaluate the correlation between TMSB10 expression and LNM of PTC.
By real-time polymerase chain reaction analysis, the expression of TMSB10 messenger RNA in normal thyroid tissue, PTC without LNM, and PTC with LNM tissue were significantly different (P < 0.0001). On immunohistochemistry analysis of 130 patients with PTC, in which 91 cases had cervical LNM and 69 cases had central neck LNM, high expression levels for TMSB10 were more common in patients with cervical LNM compared with patients without (81% versus 33%, P < 0.001). Similarly, high expression levels of TMSB10 were more common in patients with central neck LNM compared with those without (87.0% versus 44.3%, P < 0.001).
High expression levels of TMSB10 correlated with LNM in PTC, especially in the central neck region. Patients with PTC with low levels of TMSB10 expression may be unlikely to have central neck LNM and could therefore avoid prophylactic central neck dissection.
胸腺素β10（TMSB10）最近被认为是包括肿瘤血管生成、侵袭和转移在内的转移级联反应中的重要参与者。然而，该蛋白在甲状腺乳头状癌（PTC）中的作用尚未明确。
采用实时聚合酶链反应检测36例甲状腺组织样本中TMSB10信使核糖核酸的表达，这些样本包括正常甲状腺组织、无淋巴结转移（LNM）的PTC和有LNM的PTC（每个亚组12例）。免疫组织化学方面，选取2004年至2005年期间的130例PTC患者，其中91例有LNM，39例无LNM。应用统计学分析评估TMSB10表达与PTC的LNM之间的相关性。
通过实时聚合酶链反应分析，正常甲状腺组织、无LNM的PTC组织和有LNM的PTC组织中TMSB10信使核糖核酸的表达存在显著差异（P<0.0001）。对130例PTC患者进行免疫组织化学分析，其中91例有颈部LNM，69例有中央区颈部LNM，有颈部LNM的患者中TMSB10高表达水平比无颈部LNM的患者更常见（81%对33%，P<0.001）。同样，有中央区颈部LNM的患者中TMSB10高表达水平比无中央区颈部LNM的患者更常见（87.0%对44.3%，P<0.001）。
TMSB10高表达与PTC的LNM相关，尤其是在中央区颈部。TMSB10表达水平低的PTC患者可能不太可能有中央区颈部LNM，因此可以避免预防性中央区颈部清扫术。
