Xiao Ruihai, Shen Shaochen, Yu Yi, Pan Qiufeng, Kuang Renrui, Huang Hongwei
Department of Urology, The Second Affiliated Hospital of Nanchang University Nanchang, Jiangxi Province, People's Republic of China.
Int J Clin Exp Pathol. 2019 Jan 1;12(1):305-312. eCollection 2019.
The study aims to examine the effect of thymosin β10 (TMSB10) on renal cell carcinoma (RCC) progression and metastasis.
Real-time PCR and immunohistochemistry analysis were used to evaluate TMSB10 expression in RCC tissue samples and renal cancer cells. Statistical analyses were applied to investigate the association between TMSB10 expression and the clinicopathological characteristics and prognosis of RCC patients. In vitro migration and invasion assays were performed in 786-O and ACHN cells.
The expression of TMSB10 was significantly higher in renal cancer cells and tissues compared with normal kidney cells and tissues. TMSB10 expression was significantly related to tumor stage (P=0.002), lymph node metastasis (P=0.034), and distant metastasis (P=0.039). Kaplan-Meier analysis suggested that high TMSB10 expression was significantly associated with unfavorable overall (P=0.004) and recurrent-free survival (P=0.025) of RCC patients. Furthermore, TMSB10 knockdown inhibited the migration and invasion abilities of renal cancer cells in vitro.
TMSB10 is overexpressed in RCC and regulates malignant cell metastasis by inducing epithelial-mesenchymal transition, which makes TMSB10 a candidate therapeutic target for RCC.
本研究旨在探讨胸腺素β10（TMSB10）对肾细胞癌（RCC）进展和转移的影响。
采用实时荧光定量PCR和免疫组织化学分析评估RCC组织样本及肾癌细胞中TMSB10的表达。应用统计学分析研究TMSB10表达与RCC患者临床病理特征及预后的相关性。在786-O和ACHN细胞中进行体外迁移和侵袭实验。
与正常肾细胞和组织相比，肾癌细胞和组织中TMSB10的表达显著更高。TMSB10表达与肿瘤分期（P = 0.002）、淋巴结转移（P = 0.034）和远处转移（P = 0.039）显著相关。Kaplan-Meier分析表明，高TMSB10表达与RCC患者不良的总生存期（P = 0.004）和无复发生存期（P = 0.025）显著相关。此外，TMSB10基因敲低抑制了肾癌细胞的体外迁移和侵袭能力。
TMSB10在RCC中过表达，并通过诱导上皮-间质转化调节恶性细胞转移，这使得TMSB10成为RCC的一个候选治疗靶点。
