Tissue Engineering Lab, Department of Bio-Mechatronic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University, Suwon, South Korea.
Tissue Engineering Lab, Department of Bio-Mechatronic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University, Suwon, South Korea.
J Colloid Interface Sci. 2014 Sep 15;430:315-25. doi: 10.1016/j.jcis.2014.05.065. Epub 2014 Jun 10.
Biomedical scaffolds have been widely investigated because they are essential for support and promotion of cell adhesion, proliferation and differentiation in three-dimensional (3D) structures. An ideal scaffold should be highly porous to enable efficient nutrient and oxygen transfer and have a 3D structure that provides optimal micro-environmental conditions for the seeded cells to obtain homogeneous growth after a long culture period. In this study, new hierarchical osteoblast-like cell (MG-63)-laden scaffolds consisting of micro-sized struts/inter-layered micro-nanofibres and cell-laden hydrogel struts with mechanically stable and biologically superior properties were introduced. Poly(ethylene oxide) (PEO) was used as a sacrificial component to generate pores within the cell-laden hydrogel struts to attain a homogeneous cell distribution and rapid cell growth in the scaffold interior. The alginate-based cell-laden struts with PEO induced fast/homogeneous cell release, in contrast to nonporous cell-laden struts. Various weight fractions (0.5, 1, 2, 3 and 3.5 wt%) of PEO were used, of which 2 wt% PEO in the cell-laden strut resulted in the most appropriate cell release and enhanced biological activities (cell proliferation and calcium deposition), compared to nonporous cell-laden struts.
生物医学支架已被广泛研究，因为它们对于支持和促进三维（3D）结构中的细胞黏附、增殖和分化是必不可少的。理想的支架应该具有高度多孔性，以实现有效的营养物质和氧气传递，并具有 3D 结构，为接种细胞提供最佳的微环境条件，以实现长期培养后的均匀生长。在这项研究中，引入了新的分层成骨细胞样细胞（MG-63）负载支架，由微尺寸支柱/层间微纳米纤维和细胞负载水凝胶支柱组成，具有机械稳定和生物优越的特性。聚环氧乙烷（PEO）被用作牺牲成分，在细胞负载水凝胶支柱内产生孔，以实现均匀的细胞分布和支架内部的快速细胞生长。与非多孔细胞负载支柱相比，基于海藻酸盐的细胞负载支柱与 PEO 一起诱导快速/均匀的细胞释放。使用了各种重量分数（0.5、1、2、3 和 3.5wt%）的 PEO，其中细胞负载支柱中的 2wt%PEO 与非多孔细胞负载支柱相比，导致最适宜的细胞释放和增强的生物活性（细胞增殖和钙沉积）。
