富含羟脯氨酸糖蛋白在烟草（Nicotiana tabacum L.）花粉管和花柱细胞生长中的作用。

Roles of hydroxyproline-rich glycoproteins in the pollen tube and style cell growth of tobacco (Nicotiana tabacum L.).

作者信息

Zhang Xuelian, Ma Haoli, Qi Huandong, Zhao Jie

机构信息

State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, China.

出版信息

J Plant Physiol. 2014 Jul 15;171(12):1036-45. doi: 10.1016/j.jplph.2014.02.010. Epub 2014 Mar 27.

DOI:10.1016/j.jplph.2014.02.010

PMID:24974330

Abstract

Hydroxyproline-rich glycoproteins (HRGPs) are plant cell wall proteins related to plant growth and development, and extensins (EXTs) are a subfamily of HRGPs. In this study, the function of HRGPs, especially EXTs, was investigated in the pollen tube and style cell growth of Nicotiana tabacum L. By using the techniques of protein blot and immunohistochemistry, the JIM20-recognized epitopes of EXTs were abundantly expressed in vivo for pollen tubes and transmitting tissue. A hydroxyproline synthesis inhibitor, 3,4-dehydro-l-proline (3,4-DHP), was used to investigate the functions of HRGPs. The addition of 3,4-DHP decreased the speed of pollen tube growth and shortened the length of style. Moreover, the hydroxyproline assay and JIM20 immunolocalization confirmed that 3,4-DHP treatment reduced the level of hydroxyproline and EXTs in the treated styles, respectively. These results indicate that HRGPs, most likely EXTs, may play important roles in the pollen tube and style cell growth.

摘要

富含羟脯氨酸的糖蛋白（HRGPs）是与植物生长发育相关的植物细胞壁蛋白，而伸展蛋白（EXTs）是HRGPs的一个亚家族。在本研究中，对HRGPs，尤其是EXTs在烟草花粉管和花柱细胞生长中的功能进行了研究。通过蛋白质印迹和免疫组织化学技术，EXTs的JIM20识别表位在花粉管和传递组织中大量表达。使用一种羟脯氨酸合成抑制剂3,4-脱氢-L-脯氨酸（3,4-DHP）来研究HRGPs的功能。添加3,4-DHP降低了花粉管生长速度并缩短了花柱长度。此外，羟脯氨酸测定和JIM20免疫定位证实，3,4-DHP处理分别降低了处理花柱中羟脯氨酸和EXTs的水平。这些结果表明，HRGPs，很可能是EXTs，可能在花粉管和花柱细胞生长中起重要作用。