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Activation of atrial muscarinic K+ channels by low concentrations of beta gamma subunits of rat brain G protein.

作者信息

Kurachi Y, Ito H, Sugimoto T, Katada T, Ui M

机构信息

2nd Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan.

出版信息

Pflugers Arch. 1989 Jan;413(3):325-7. doi: 10.1007/BF00583550.

DOI:10.1007/BF00583550
PMID:2497438
Abstract

Effects of G protein beta gamma subunits from rat brain on cardiac K+ channel was examined in single atrial cells of guinea-pig, using patch clamp techniques. We found that 10 pM concentration of rat brain beta gamma subunits preparation could activate the atrial muscarine receptor-gated K+ channel (IK.ACh). Neither the detergent, CHAPS, used to suspend beta gamma nor the boiled beta gamma preparation activated IK.ACh. Furthermore, preincubation of beta gamma subunits preparation in Mg2+-free solution, which easily inactivated alpha-GTP-gamma S, did not affect beta gamma-activation of IK.ACh. We concluded, therefore, that beta gamma subunits themselves can activate IK.ACh.

摘要

相似文献

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2
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4
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本文引用的文献

1
Effects of guanine nucleotides and Mg on human erythrocyte Ni and Ns, the regulatory components of adenylyl cyclase.鸟嘌呤核苷酸和镁对人红细胞腺苷酸环化酶的调节成分Ni和Ns的影响。
J Biol Chem. 1984 Sep 25;259(18):11408-18.
2
A novel mechanism for the inhibition of adenylate cyclase via inhibitory GTP-binding proteins. Calmodulin-dependent inhibition of the cyclase catalyst by the beta gamma-subunits of GTP-binding proteins.一种通过抑制性GTP结合蛋白抑制腺苷酸环化酶的新机制。GTP结合蛋白的βγ亚基对环化酶催化剂的钙调蛋白依赖性抑制。
J Biol Chem. 1987 Sep 5;262(25):11897-900.
3
Stimulation of phospholipase A2 activity in bovine rod outer segments by the beta gamma subunits of transducin and its inhibition by the alpha subunit.
Biophys J. 2013 Sep 17;105(6):1515-25. doi: 10.1016/j.bpj.2013.08.009.
4
Phosphatidylinositol 3,4,5-trisphosphate and Ca2+/calmodulin competitively bind to the regulators of G-protein-signalling (RGS) domain of RGS4 and reciprocally regulate its action.磷脂酰肌醇3,4,5-三磷酸与Ca2+/钙调蛋白竞争性结合RGS4的G蛋白信号调节因子（RGS）结构域，并相互调节其作用。
Biochem J. 2005 Jan 1;385(Pt 1):65-73. doi: 10.1042/BJ20040404.
5
Cell signal control of the G protein-gated potassium channel and its subcellular localization.G蛋白门控钾通道的细胞信号调控及其亚细胞定位。
J Physiol. 2004 Jan 15;554(Pt 2):285-94. doi: 10.1113/jphysiol.2003.048439. Epub 2003 Aug 15.
6
Expression of an atrial G-protein-activated potassium channel in Xenopus oocytes.心房G蛋白激活钾通道在非洲爪蟾卵母细胞中的表达。
Proc Natl Acad Sci U S A. 1993 Jul 15;90(14):6596-600. doi: 10.1073/pnas.90.14.6596.
7
G proteins: critical control points for transmembrane signals.G蛋白：跨膜信号的关键控制点。
Protein Sci. 1994 Jan;3(1):3-14. doi: 10.1002/pro.5560030102.
8
A G protein-gated K channel is activated via beta 2-adrenergic receptors and G beta gamma subunits in Xenopus oocytes.在非洲爪蟾卵母细胞中，一种G蛋白门控钾通道通过β2 - 肾上腺素能受体和Gβγ亚基被激活。
J Gen Physiol. 1995 Mar;105(3):421-39. doi: 10.1085/jgp.105.3.421.
9
Evidence that neuronal G-protein-gated inwardly rectifying K+ channels are activated by G beta gamma subunits and function as heteromultimers.有证据表明神经元G蛋白门控内向整流钾通道由Gβγ亚基激活并作为异源多聚体发挥作用。
Proc Natl Acad Sci U S A. 1995 Jul 3;92(14):6542-6. doi: 10.1073/pnas.92.14.6542.
10
Guanine nucleotide-binding protein, alpha i-3, directly activates a cation channel in rat renal inner medullary collecting duct cells.鸟嘌呤核苷酸结合蛋白αi-3直接激活大鼠肾内髓集合管细胞中的阳离子通道。
J Clin Invest. 1989 Jul;84(1):352-6. doi: 10.1172/JCI114162.
转导蛋白的βγ亚基对牛视杆细胞外段磷脂酶A2活性的刺激作用及其α亚基对该活性的抑制作用。
Proc Natl Acad Sci U S A. 1987 Jun;84(11):3623-7. doi: 10.1073/pnas.84.11.3623.
4
A new GTP-binding protein in brain tissues serving as the specific substrate of islet-activating protein, pertussis toxin.一种存在于脑组织中的新型GTP结合蛋白，它是百日咳毒素（胰岛激活蛋白）的特异性底物。
FEBS Lett. 1987 Mar 23;213(2):353-8. doi: 10.1016/0014-5793(87)81521-1.
5
Two guanine nucleotide-binding proteins in rat brain serving as the specific substrate of islet-activating protein, pertussis toxin. Interaction of the alpha-subunits with beta gamma-subunits in development of their biological activities.大鼠脑中作为百日咳毒素（胰岛激活蛋白）特异性底物的两种鸟嘌呤核苷酸结合蛋白。α亚基与βγ亚基在其生物活性发展过程中的相互作用。
J Biol Chem. 1986 Jun 25;261(18):8182-91.
6
Specificity of action of guanine nucleotide-binding regulatory protein subunits on the cardiac muscarinic K+ channel.鸟嘌呤核苷酸结合调节蛋白亚基对心肌毒蕈碱钾通道的作用特异性
Proc Natl Acad Sci U S A. 1988 Aug;85(16):5814-8. doi: 10.1073/pnas.85.16.5814.
7
Alpha-subunit of Gk activates atrial K+ channels of chick, rat, and guinea pig.
Am J Physiol. 1988 Jun;254(6 Pt 2):H1200-5. doi: 10.1152/ajpheart.1988.254.6.H1200.
8
G protein opening of K+ channels.G蛋白介导的钾通道开放。
Nature. 1987;327(6117):21-2. doi: 10.1038/327021a0.
9
The alpha subunit of the GTP binding protein Gk opens atrial potassium channels.GTP结合蛋白Gk的α亚基可开启心房钾通道。
Science. 1987 Apr 24;236(4800):442-5. doi: 10.1126/science.2436299.
10
The beta gamma subunits of GTP-binding proteins activate the muscarinic K+ channel in heart.GTP结合蛋白的βγ亚基激活心脏中的毒蕈碱型钾通道。
Nature. 1987;325(6102):321-6. doi: 10.1038/325321a0.