Nii Daisuke, Hayashida Takuya, Yamaguchi Yuuki, Ikawa Shukuko, Shibata Takehiko, Umemura Kazuo
Biophysics Section, Department of Physics, Graduate School of Science, Tokyo University of Science, 1-3 Kagurazaka, Shinjuku, Tokyo 1628601, Japan.
Cellular and Molecular Biology Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan; Graduate School of Nanobioscience, Yokohama City University, 1-7-29 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan.
Colloids Surf B Biointerfaces. 2014 Sep 1;121:325-30. doi: 10.1016/j.colsurfb.2014.06.008. Epub 2014 Jun 10.
Single-stranded DNA-binding (SSB) proteins were treated with hybrids of DNA and single-walled carbon nanotubes (SWNTs) to examine the biological function of the DNA molecules adsorbed on the SWNT surface. When single-stranded DNA (ssDNA) was used for the hybridization, significant binding of the SSB molecules to the ssDNA-SWNT hybrids was observed by using atomic force microscopy (AFM) and agarose gel electrophoresis. When double-stranded DNA (dsDNA) was used, the SSB molecules did not bind to the dsDNA-SWNT hybrids in most of the conditions that we evaluated. A specifically modified electrophoresis procedure was used to monitor the locations of the DNA, SSB, and SWNT molecules. Our results clearly showed that ssDNA/dsDNA molecules on the SWNT surfaces retained their single-stranded/double-stranded structures.
将单链DNA结合（SSB）蛋白与DNA和单壁碳纳米管（SWNT）的杂交体进行处理，以研究吸附在SWNT表面的DNA分子的生物学功能。当使用单链DNA（ssDNA）进行杂交时，通过原子力显微镜（AFM）和琼脂糖凝胶电泳观察到SSB分子与ssDNA-SWNT杂交体有显著结合。当使用双链DNA（dsDNA）时，在我们评估的大多数条件下，SSB分子不与dsDNA-SWNT杂交体结合。采用一种经过特殊改良的电泳程序来监测DNA、SSB和SWNT分子的位置。我们的结果清楚地表明，SWNT表面的ssDNA/dsDNA分子保留了它们的单链/双链结构。
