Iwasa Takeshi, Matsuzaki Toshiya, Matsui Sumika, Munkhzaya Munkhsaikhan, Tungalagsuvd Altankhuu, Kawami Takako, Murakami Masahiro, Kato Takeshi, Kuwahara Akira, Yasui Toshiyuki, Irahara Minoru
Department of Obstetrics and Gynecology, The University of Tokushima Graduate School, Institute of Health Biosciences, Tokushima 770-8503, Japan.
Endocr J. 2014;61(9):891-900. doi: 10.1507/endocrj.ej14-0042. Epub 2014 Jun 29.
Adiponectin (APN), secreted by white adipose tissue (WAT), acts as a protective factor against inflammatory conditions. However, the changes in the expression levels of endogenous APN and the two types of APN receptor (AdipoR1 and AdipoR2) induced by acute inflammatory conditions have not been fully elucidated. In this study, the changes in peripheral and/or central APN and AdipoR expression caused by lipopolysaccharide (LPS)-induced sepsis were examined in gonadal-intact (Sham) and ovariectomized (OVX) female rats. As it has been reported that APN and AdipoR suppress the production of inflammatory cytokines to prevent excessive inflammation, the mRNAs of these molecules were also examined. LPS injection induced increases in visceral WAT APN mRNA without affecting the serum APN level in both the Sham and OVX rats. OVX rats exhibited higher serum APN levels than Sham rats. LPS injection increased the subcutaneous WAT APN mRNA in OVX rats. In both Sham and OVX rats, LPS injection led to a decrease in hepatic AdipoR2 mRNA and an increase in hypothalamic AdipoR2 mRNA. Hypothalamic AdipoR2 mRNA was upregulated 24 h after LPS injection in OVX but not Sham rats. Serum TNF-α level at 6 h after LPS injection and hypothalamic and hepatic IL-6 and TNF-α mRNA at 24 h after LPS injection were significantly higher in Sham than OVX rats. These results suggest that APN and AdipoR play roles in modulating inflammation under septic conditions in female rats.
脂联素（APN）由白色脂肪组织（WAT）分泌，作为一种针对炎症状态的保护因子发挥作用。然而，急性炎症状态诱导的内源性APN及两种类型的APN受体（AdipoR1和AdipoR2）表达水平的变化尚未完全阐明。在本研究中，在性腺完整（假手术组）和去卵巢（OVX）雌性大鼠中检测了脂多糖（LPS）诱导的脓毒症引起的外周和/或中枢APN及AdipoR表达的变化。由于已有报道称APN和AdipoR可抑制炎性细胞因子的产生以防止过度炎症，因此也检测了这些分子的mRNA。LPS注射诱导假手术组和OVX组大鼠内脏WAT APN mRNA增加，但不影响血清APN水平。OVX大鼠的血清APN水平高于假手术组大鼠。LPS注射使OVX大鼠皮下WAT APN mRNA增加。在假手术组和OVX组大鼠中，LPS注射均导致肝脏AdipoR2 mRNA减少，下丘脑AdipoR2 mRNA增加。LPS注射后24小时，OVX大鼠下丘脑AdipoR2 mRNA上调，而假手术组大鼠未出现这种情况。LPS注射后6小时血清TNF-α水平以及LPS注射后24小时下丘脑和肝脏IL-6及TNF-α mRNA水平在假手术组大鼠中显著高于OVX大鼠。这些结果表明，APN和AdipoR在雌性大鼠脓毒症状态下调节炎症中发挥作用。
