Iwasa Takeshi, Matsuzaki Toshiya, Matsui Sumika, Tungalagsuvd Altankhuu, Munkhzaya Munkhsaikhan, Takiguchi Eri, Kawakita Takako, Kuwahara Akira, Yasui Toshiyuki, Irahara Minoru
Department of Obstetrics and Gynecology, The University of Tokushima Graduate School, Institute of Health Biosciences, 3-18-15 Kuramoto-Cho, Tokushima 770-8503, Japan.
Department of Obstetrics and Gynecology, The University of Tokushima Graduate School, Institute of Health Biosciences, 3-18-15 Kuramoto-Cho, Tokushima 770-8503, Japan.
Int Immunopharmacol. 2016 Jun;35:243-247. doi: 10.1016/j.intimp.2016.04.002. Epub 2016 Apr 16.
Visfatin plays an important role in inflammatory and metabolic conditions. In this study, the effects of septic stress on the serum, white-adipose-tissue (WAT), and liver visfatin levels of male and female rats were examined. Both gonadally intact (sham) and ovariectomized (OVX) female rats were used in order to evaluate the effects of the gonadal hormonal milieu on visfatin responses. Under the saline-injected conditions, the serum visfatin levels and the hepatic, subcutaneous, and visceral WAT visfatin mRNA levels of the OVX and sham rats did not differ. The serum visfatin levels and the subcutaneous, visceral WAT, and hepatic visfatin mRNA levels of both male and female rats were increased by the injection of a septic dose (5mg/kg) of LPS. At 6h after the injection of LPS, the WAT visfatin mRNA levels of the OVX rats were higher than those of the sham rats, whereas the serum visfatin levels and hepatic visfatin mRNA levels of the two groups did not differ. In the cultured visceral WAT, visfatin antagonist (FK-866) attenuated the LPS-induced up-regulations of pro-inflammatory cytokines (IL-1β, IL-6, and TNF-α). The pathophysiological roles of visfatin under septic conditions remain to be clarified. In addition, the precise mechanisms responsible for the increased WAT visfatin expression seen after ovariectomy and the effects of such changes should also be clarified.
内脂素在炎症和代谢状况中发挥着重要作用。在本研究中，检测了脓毒症应激对雄性和雌性大鼠血清、白色脂肪组织（WAT）及肝脏内脂素水平的影响。为评估性腺激素环境对内脂素反应的影响，使用了性腺完整（假手术）和去卵巢（OVX）的雌性大鼠。在注射生理盐水的条件下，OVX大鼠和假手术大鼠的血清内脂素水平以及肝脏、皮下和内脏WAT内脂素mRNA水平并无差异。注射脓毒症剂量（5mg/kg）的脂多糖（LPS）可使雄性和雌性大鼠的血清内脂素水平以及皮下、内脏WAT和肝脏内脂素mRNA水平升高。注射LPS后6小时，OVX大鼠的WAT内脂素mRNA水平高于假手术大鼠，而两组的血清内脂素水平和肝脏内脂素mRNA水平并无差异。在培养的内脏WAT中，内脂素拮抗剂（FK-866）可减弱LPS诱导的促炎细胞因子（IL-1β、IL-6和TNF-α）的上调。脓毒症条件下内脂素的病理生理作用仍有待阐明。此外，去卵巢后WAT内脂素表达增加的具体机制及其变化的影响也应予以阐明。
