Bogen B
Institute for Immunology and Rheumatology, University of Oslo, Norway.
Scand J Immunol. 1989 Mar;29(3):273-9. doi: 10.1111/j.1365-3083.1989.tb01125.x.
Rat monoclonal antibodies directed against the BALB/c myeloma protein M315 (alpha,lambda 2) are described. 9A8 (IgG1) binds the V domain of lambda 2 and cross-reacts with lambda 1 and lambda 3 chains. 2B6 (IgG2a) is directed to the C domain of lambda 2 and cross-reacts with C lambda 3. The antibodies bind isolated chains as well as complete immunoglobulins. The monoclonals detect soluble immunoglobulin (radioimmunoassay), immunoglobulin immobilized on polystyrene (enzyme-linked immunosorbent assay), immunoglobulin bound to nitrocellulose (immunoblotting), and surface immunoglobulin intercalated in cell membranes (immunofluorescence). The antibodies are easily purified on protein G immunosorbents and may be biotinylated or conjugated with fluorescein isothiocyanate without loss of capacity to bind. In addition to the anti-lambda antibodies, a C alpha 2/C alpha 3-specific monoclonal antibody, 8D2 (IgG2a) is described.
本文描述了针对BALB/c骨髓瘤蛋白M315（α，λ2）的大鼠单克隆抗体。9A8（IgG1）结合λ2的V结构域，并与λ1和λ3链发生交叉反应。2B6（IgG2a）针对λ2的C结构域，并与Cλ3发生交叉反应。这些抗体既能结合分离的链，也能结合完整的免疫球蛋白。这些单克隆抗体可检测可溶性免疫球蛋白（放射免疫测定）、固定在聚苯乙烯上的免疫球蛋白（酶联免疫吸附测定）、与硝酸纤维素结合的免疫球蛋白（免疫印迹）以及插入细胞膜中的表面免疫球蛋白（免疫荧光）。这些抗体很容易在蛋白G免疫吸附剂上纯化，并且可以生物素化或与异硫氰酸荧光素偶联，而不会丧失结合能力。除了抗λ抗体外，还描述了一种Cα2/Cα3特异性单克隆抗体8D2（IgG2a）。
