Zhou D, Leslie G A, Guo K, Gutman G A
J Immunogenet. 1986 Aug;13(4):299-307. doi: 10.1111/j.1744-313x.1986.tb01114.x.
We immunized a BALB/c mouse with the lambda-bearing rat IgG1 myeloma IR31, fused its spleen cells with the hybridoma parent line P3.X63.Ag8.653, and isolated a monoclonal antibody (G33/11) directed against rat immunoglobulin lambda chains. We used this antibody to classify two existing rat hybridomas as lambda-bearing proteins (D4.37HL.252 and PC61.5), and isolated one new lambda-bearing rat IgM hybridoma, G36/1. All the normal inbred rat sera that were tested contained lambda-bearing Ig as detected by G33/11, at levels ranging from 1.5% to 13% of the total serum Ig, the mean value being 7.9%. This antibody will be valuable for broadening our understanding of the immunogenetics of the rat, and for the characterization of monoclonal antibodies made in this species.
我们用携带λ链的大鼠IgG1骨髓瘤IR31免疫一只BALB/c小鼠，将其脾细胞与杂交瘤亲本系P3.X63.Ag8.653融合，并分离出一种针对大鼠免疫球蛋白λ链的单克隆抗体（G33/11）。我们用该抗体将两个现有的大鼠杂交瘤分类为携带λ链的蛋白（D4.37HL.252和PC61.5），并分离出一个新的携带λ链的大鼠IgM杂交瘤G36/1。所有检测的正常近交系大鼠血清中，用G33/11检测均含有携带λ链的Ig，其水平占总血清Ig的1.5%至13%，平均值为7.9%。该抗体对于拓宽我们对大鼠免疫遗传学的理解以及对该物种产生的单克隆抗体的表征将具有重要价值。
