Sánchez-Dávila F, Ledezma-Torres R A, Padilla-Rivas G, Del Bosque-González A S, González Gómez A, Bernal-Barragán H
Universidad Autonoma de Nuevo Leon, Facultad de Agronomia, Nuevo Leon, Mexico.
Universidad Autonoma de Nuevo Leon, Facultad de Medicina Veterinaria y Zootecnia, Nuevo Leon, Mexico.
Reprod Domest Anim. 2014 Aug;49(4):e40-e43. doi: 10.1111/rda.12350. Epub 2014 Jun 30.
The aim of this research was to evaluate the effectiveness of three pFSH doses (80 mg; 145 mg and 215 mg) on ovarian response and on quantity and quality of transferable embryos of goats during the breeding and the non-breeding seasons. Ovary structures were exposed (laparatomy under general anaesthesia) and numbers of follicles and corpora lutea were registered. Surgical embryo flushing was conducted to count and classify embryos. There were more follicles (3.4 ± 1.1) in does administered 80 mg of pFSH (p < 0.05) than in goats administered 145 mg of pFSH (2.2 ± 1.1) and 215 mg of pFSH (0.9 ± 0.6). Numbers of corpora lutea, blastocysts, and recovered and transferable embryos of goats administered 145 mg pFSH (13.4 ± 3.7, 2.42 ± 1.0, 3.4 ± 1.2 and 3.2 ± 1.1, respectively) and those of goats administered 215 mg pFSH (11.6 ± 2.6, 3.2 ± 0.9, 5.7 ± 1.5, and 5.6 ± 1.5) were greater (p < 0.05) than values obtained from goats administered 80 mg pFSH (4.0 ± 1.5, 0.5 ± 0.3, 1.0 ± 0.5, and 0.8 ± 0.5). Numbers of morula of does administered 80 and 145 mg pFSH (0.4 ± 0.4 and 0.8 ± 0.3) were lower (p < 0.05) than those obtained from animals treated with 215 mg pFSH (2.4 ± 0.9). There was no effect of season upon the analyzed variables. In conclusion, under the prevalent conditions in north-eastern Mexico, administration of 145 or 215 mg pFSH in a decreasing dose schedule over 3.5 days to bred goats provided a satisfactory superovulatory result.
本研究的目的是评估三种促卵泡素(pFSH)剂量(80毫克、145毫克和215毫克)对山羊在繁殖季节和非繁殖季节的卵巢反应以及可移植胚胎数量和质量的影响。暴露卵巢结构(全身麻醉下进行剖腹术)并记录卵泡和黄体数量。进行手术胚胎冲洗以计数和分类胚胎。接受80毫克pFSH的母羊卵泡数量(3.4±1.1)比接受145毫克pFSH的山羊(2.2±1.1)和接受215毫克pFSH的山羊(0.9±0.6)更多(p<0.05)。接受145毫克pFSH的山羊的黄体、囊胚以及回收和可移植胚胎数量(分别为13.4±3.7、2.42±1.0、3.4±1.2和3.2±1.1)和接受215毫克pFSH的山羊(11.6±2.6、3.2±0.9、5.7±1.5和5.6±1.5)比接受80毫克pFSH的山羊(4.0±1.5、0.5±0.3、1.0±0.5和0.8±0.5)的值更高(p<0.05)。接受80毫克和145毫克pFSH的母羊桑椹胚数量(0.4±0.4和0.8±0.3)比接受215毫克pFSH处理的动物(2.4±0.9)更低(p<0.05)。季节对分析变量没有影响。总之,在墨西哥东北部的普遍条件下,对已配种山羊在3.5天内按递减剂量方案给予145或215毫克pFSH可获得令人满意的超数排卵结果。