Leston J, Harthé C, Mottolese C, Mertens P, Sindou M, Claustrat B
Neurosurgical Unit A, P.-Wertheimer Hospital, Bron, France; Inserm, U846, Department of Chronobiology, Stem Cell and Brain Research Institute, Bron, France.
Hormone Laboratory, Centre of Nuclear Medicine, Department of Chronobiology, Stem Cell and Brain Research Institute, Groupement Hospitalier Est, 59, boulevard Pinel, 69677 Bron, France.
Neurochirurgie. 2015 Apr-Jun;61(2-3):85-9. doi: 10.1016/j.neuchi.2013.04.004. Epub 2014 Jun 26.
In order to determine sources and metabolism of melatonin in human cerebrospinal fluid (CSF), melatonin and 6-sulfatoxymelatonin (aMT6S) concentrations were measured in CSF sampled during neurosurgery in both lateral and third ventricles in patients displaying movement disorder (Parkinson's disease, essential tremor, dystonia or dyskinesia) and compared with their plasma levels. Previous determinations in nocturnal urine had showed that the patients displayed melatonin excretion in the normal range, compared with healthy controls matched according to age. A significant difference in melatonin concentration was observed between lateral and third ventricles, with the highest levels in the third ventricle (8.75±2.75pg/mL vs. 3.20±0.33pg/mL, P=0.01). CSF aMT6s levels were similar in both ventricles and of low magnitude, less than 5pg/mL. They were not correlated with melatonin levels or influenced by the area of sampling. Melatonin levels were significantly higher in third ventricle than in the plasma, whereas there was no difference between plasma and lateral ventricle levels. These findings show that melatonin may enter directly the CSF through the pineal recess in humans. The physiological meaning of these data remains to be elucidated.
为了确定人脑脊液（CSF）中褪黑素的来源和代谢情况，在神经外科手术期间，对患有运动障碍（帕金森病、特发性震颤、肌张力障碍或运动障碍）的患者侧脑室和第三脑室采集的脑脊液中的褪黑素和6-硫酸氧褪黑素（aMT6S）浓度进行了测量，并与他们的血浆水平进行比较。先前对夜间尿液的检测表明，与按年龄匹配的健康对照相比，这些患者的褪黑素排泄处于正常范围。在侧脑室和第三脑室之间观察到褪黑素浓度存在显著差异，第三脑室中的水平最高（8.75±2.75pg/mL对3.20±0.33pg/mL，P = 0.01）。两个脑室中的脑脊液aMT6S水平相似且较低，低于5pg/mL。它们与褪黑素水平无关，也不受采样区域的影响。第三脑室中的褪黑素水平明显高于血浆中的水平，而血浆和侧脑室水平之间没有差异。这些发现表明，褪黑素可能通过松果体隐窝直接进入人脑脊液。这些数据的生理意义仍有待阐明。
