Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety.
Nanoscale. 2014 Aug 7;6(15):8579-84. doi: 10.1039/c4nr02062f.
We report single-molecule detection of Hg(2+) by threading an Hg(2+)-mediated DNA duplex through α-hemolysin nanopores, which generates characteristic three-level current patterns and enables the unambiguous detection of Hg(2+). This strategy precludes any background interference and features high sensitivity and selectivity. Moreover, the platform could be readily integrated with aptamers and molecular beacons, offering extended possibilities for the construction of a new DNA-based nanopore sensing system.
我们通过将 Hg(2+)-介导的 DNA 双链体穿过α-溶血素纳米孔来实现对 Hg(2+)的单分子检测，该方法产生特征性的三级电流模式，从而能够明确地检测 Hg(2+)。该策略排除了任何背景干扰，具有高灵敏度和选择性。此外，该平台可以与适体和分子信标轻松集成，为构建新的基于 DNA 的纳米孔传感系统提供了更多可能。
